Department of Solar Materials, Helmholtz-Centre for Environmental Research - UFZ, Leipzig, Germany.
Faculty of Biology, Genetics and Experimental Bioinformatics, University of Freiburg, Freiburg, Germany.
Biol Direct. 2021 Dec 14;16(1):26. doi: 10.1186/s13062-021-00316-4.
Microorganisms evolved specific acclimation strategies to thrive in environments of high or fluctuating salinities. Here, salt acclimation in the model cyanobacterium Synechocystis sp. PCC 6803 was analyzed by integrating transcriptomic, proteomic and metabolomic data. A dynamic reorganization of the transcriptome occurred during the first hours after salt shock, e.g. involving the upregulation of genes to activate compatible solute biochemistry balancing osmotic pressure. The massive accumulation of glucosylglycerol then had a measurable impact on the overall carbon and nitrogen metabolism. In addition, we observed the coordinated induction of putative regulatory RNAs and of several proteins known for their involvement in other stress responses. Overall, salt-induced changes in the proteome and transcriptome showed good correlations, especially among the stably up-regulated proteins and their transcripts. We define an extended salt stimulon comprising proteins directly or indirectly related to compatible solute metabolism, ion and water movements, and a distinct set of regulatory RNAs involved in post-transcriptional regulation. Our comprehensive data set provides the basis for engineering cyanobacterial salt tolerance and to further understand its regulation.
微生物进化出了特定的适应策略，以在高盐或盐度波动的环境中茁壮成长。在这里，通过整合转录组学、蛋白质组学和代谢组学数据，分析了模式蓝藻集胞藻 PCC 6803 的盐度适应。在盐冲击后的最初几个小时内，转录组发生了动态重组，例如涉及上调基因以激活兼容溶质生物化学平衡渗透压。然后，大量积累的葡糖基甘油对整体碳氮代谢产生了可衡量的影响。此外，我们观察到推测的调节 RNA 和几种已知参与其他应激反应的蛋白质的协调诱导。总体而言，盐诱导的蛋白质组和转录组变化显示出良好的相关性，特别是在稳定上调的蛋白质及其转录物之间。我们定义了一个扩展的盐刺激物组，包括直接或间接与兼容溶质代谢、离子和水运动以及一组涉及转录后调控的独特调节 RNA 相关的蛋白质。我们的综合数据集为工程蓝藻的耐盐性提供了基础，并进一步了解其调控机制。
