Sendashonga C N, Black S J
Parasite Immunol. 1986 Sep;8(5):443-53. doi: 10.1111/j.1365-3024.1986.tb00860.x.
Cells collected from C57B1/6 mice infected with monomorphic and pleomorphic clones of Trypanosoma brucei parasites (ILTat 1.4 and GUTat 3.1) were analysed for the incorporation of 125I-Iododeoxyuridine into DNA of total splenic lymphocytes and of B and T lymphocytes isolated on a fluorescence activated cell sorter. The monomorphic T. brucei ILTat 1.4 parasites triggered delayed and low splenic DNA synthetic responses in comparison to those arising in mice infected with the pleomorphic T. brucei GUTat 3.1 organisms. Mice infected with both parasite clones mounted splenic DNA synthetic responses similar to those arising in animals infected with the pleomorphic organisms alone and similar responses were induced by lethally irradiated T. bruceiGUTat 3.1 and T. brucei ILTat 1.4 parasites. In mice infected with the pleomorphic parasites, DNA synthesis was first detected in the T cell population and B cell DNA synthetic responses were detected between 1 and 2 days later. In contrast only T cell DNA synthetic responses were detected after infection with the monomorphic T. brucei ILTat 1.4 parasites. It is suggested that the previously reported failure of monomorphic T. brucei parasites to trigger antibody production in infected mice is a result of their inability to stimulate B lymphocytes.
从感染布氏锥虫寄生虫（ILTat 1.4和GUTat 3.1）单形和多形克隆的C57B1/6小鼠中收集细胞，分析125I-碘脱氧尿苷掺入总脾淋巴细胞以及在荧光激活细胞分选仪上分离的B淋巴细胞和T淋巴细胞DNA的情况。与感染多形布氏锥虫GUTat 3.1的小鼠相比，单形布氏锥虫ILTat 1.4寄生虫引发的脾DNA合成反应延迟且较低。感染两种寄生虫克隆的小鼠产生的脾DNA合成反应与仅感染多形寄生虫的动物相似，并且致死照射的布氏锥虫GUTat 3.1和布氏锥虫ILTat 1.4寄生虫也诱导了类似的反应。在感染多形寄生虫的小鼠中，首先在T细胞群体中检测到DNA合成，1至2天后检测到B细胞DNA合成反应。相比之下，感染单形布氏锥虫ILTat 1.4寄生虫后仅检测到T细胞DNA合成反应。有人提出，先前报道的单形布氏锥虫寄生虫在感染小鼠中未能引发抗体产生是由于它们无法刺激B淋巴细胞。
