Gasbarre L C, Hug K, Louis J A
Clin Exp Immunol. 1980 Jul;41(1):97-106.
A procedure which results in the specific activation of primed murine T lymphocytes was adapted for the study of T lymphocyte activation by the African trypanosome: Trypanosoma brucei. The assay calls for the in vivo priming of lymphocytes by the subcutaneous administration of parasites, followed by the co-cultivation in vitro of cells taken from the regional draining lymph nodes and the parasite. This co-cultivation results in a marked proliferation of lymphoid cells. The proliferation was shown to be specific for the parasite, and to be dependent on the presence of T lymphocytes and macrophages. Both the in vivo priming and the in vitro activation were shown to require the presence of living parasites. Various factors influencing the magnitude of the proliferative response were analysed. Of special interest is the observation that the time interval between in vivo priming and in vitro culture which results in a substantial proliferative response is quite short when compared to that seen with other antigens. Although lymph node cells from mice primed with T. brucei 1 to 2 weeks previously are able to mount a secondary proliferative response upon stimulation with T. brucei, cells taken 3 weeks after priming are unresponsive to an in vitro challenge with T. brucei. This unresponsiveness may be a result of the generalized immunosuppression seen in African trypanosomiasis. Thus, this method offers the potential for the study of specific T cell responsiveness in African trypanosome infections.
一种能导致致敏小鼠T淋巴细胞特异性激活的程序被应用于研究非洲锥虫（布氏锥虫）对T淋巴细胞的激活作用。该检测方法要求通过皮下注射寄生虫对淋巴细胞进行体内致敏，随后将取自局部引流淋巴结的细胞与寄生虫进行体外共培养。这种共培养会导致淋巴细胞显著增殖。已证明这种增殖对寄生虫具有特异性，并且依赖于T淋巴细胞和巨噬细胞的存在。体内致敏和体外激活均显示需要活的寄生虫存在。分析了影响增殖反应程度的各种因素。特别值得关注的是，与其他抗原相比，体内致敏和体外培养之间导致大量增殖反应的时间间隔相当短。虽然用布氏锥虫致敏1至2周后的小鼠淋巴结细胞在用布氏锥虫刺激时能够产生二次增殖反应，但致敏3周后获取的细胞对布氏锥虫的体外刺激无反应。这种无反应性可能是非洲锥虫病中所见的全身性免疫抑制的结果。因此，该方法为研究非洲锥虫感染中特异性T细胞反应性提供了可能性。
