Bonorden Melissa J L, Carmella Steven G, Ballinger Oliver T, Williams Jessica, Dorn Irv, Vanderloo Hanna, Fujioka Naomi, Hatsukami Dorothy K, Hecht Stephen S
Hormel Foods Corporation, Austin, Minnesota.
Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota.
Cancer Prev Res (Phila). 2022 Mar 1;15(3):143-149. doi: 10.1158/1940-6207.CAPR-21-0473.
Watercress is an excellent source of phenethyl isothiocyanate (PEITC), shown in many studies to enhance carcinogen and toxicant detoxification and to inhibit carcinogenesis. Based on a previous observation that PEITC can enhance the detoxification of common environmental pollutants such as acrolein, crotonaldehyde, benzene, and 1,3-butadiene, we designed a clinical trial testing the hypothesis that daily consumption of a drink containing freeze-dried watercress, an abundant source of PEITC, would have a similar effect, particularly observed in subjects who were null in certain glutathione S-transferase genes. This manuscript describes the preparation of nearly 100 pounds of freeze-dried watercress for this trial, starting with laboratory-scale pilot studies and proceeding to industrial-scale production of the fully validated product in compliance with all food safety requirements. Initial results validating subject compliance in the clinical trial are also presented.
This study describes the preparation of a beverage containing freeze-dried watercress suitable for consumption in a clinical trial to determine whether a constituent of this beverage-PEITC, which has cancer prevention properties-can enhance detoxification of common environmental carcinogens and toxicants such as benzene, which may have a role in environmentally induced cancer. See related Spotlight, p. 139.
水田芥是苯乙基异硫氰酸酯（PEITC）的优质来源，许多研究表明它能增强致癌物和毒物的解毒作用并抑制致癌作用。基于之前的一项观察，即PEITC可增强对常见环境污染物如丙烯醛、巴豆醛、苯和1,3 - 丁二烯的解毒作用，我们设计了一项临床试验，检验如下假设：每日饮用含有冻干水田芥（PEITC的丰富来源）的饮品会有类似效果，尤其在某些谷胱甘肽S - 转移酶基因缺失的受试者中会观察到这种效果。本手稿描述了为该试验制备近100磅冻干水田芥的过程，从实验室规模的试点研究开始，进而按照所有食品安全要求进行工业化规模生产并完全验证产品。还展示了在临床试验中验证受试者依从性的初步结果。
本研究描述了一种含有冻干水田芥的饮品的制备，该饮品适合在临床试验中饮用，以确定这种饮品的一种成分——具有防癌特性的PEITC——是否能增强对常见环境致癌物和毒物如苯的解毒作用，而苯可能在环境诱导的癌症中起作用。见相关聚焦内容，第139页。
