Suzuki T, Ikeda Y, Ijima M, Fujiki Y, Nakao K, Nakazawa T
Yakubutsu Seishin Kodo. 1986 Jun;6(2):253-8.
A sensitive and useful radioimmunoassay for CRF was established using synthetic ovine CRF. CRF-like immunoreactivities of hypothalami in the bovine, human, rat, guinea pig, and mouse could be detected with this radioimmunoassay. A variety of CRF-like immunoreactivities of hypothalami in the mammalian brains led us to discuss a putative species difference of amino-acid structure in CRF. In the bovine, CRF-like immunoreactivities of the frontal cortex, caudate nucleus, putamen, and hippocampus could be detected in addition to CRF-like immunoreactivity of hypothalamus. These observations suggested a wide distribution of CRF in the central nervous system, and gave room for discussion about a physiological role of extrahypothalamic CRF. Gel chromatographic studies revealed a high-molecular-weight form of CRF-like immunoreactivity in the bovine frontal cortical profile that was not found in the hypothalamic profile. The results show a multiplicity of CRF-like immunoreactivity in the bovine brain.
利用合成的羊促肾上腺皮质激素释放因子（CRF）建立了一种灵敏且实用的CRF放射免疫分析法。采用该放射免疫分析法可检测牛、人、大鼠、豚鼠和小鼠下丘脑的CRF样免疫反应性。哺乳动物大脑中下丘脑的多种CRF样免疫反应性促使我们探讨CRF中氨基酸结构的假定种属差异。在牛中，除下丘脑的CRF样免疫反应性外，还可检测到额叶皮质、尾状核、壳核和海马的CRF样免疫反应性。这些观察结果提示CRF在中枢神经系统中广泛分布，并为探讨下丘脑外CRF的生理作用提供了讨论空间。凝胶色谱研究显示，牛额叶皮质图谱中存在一种下丘脑图谱中未发现的高分子量形式的CRF样免疫反应性。结果表明牛脑中存在多种CRF样免疫反应性。
