School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
Mikrochim Acta. 2021 Dec 14;189(1):28. doi: 10.1007/s00604-021-05093-5.
The aggregation-induced emission (AIE) characteristic of copper nanoclusters (CuNC) was for the first time used to construct a ratiometric fluorescence probe (CuNC-Al) for detection of chlortetracycline (CTC). Aluminum ion (Al) can aggregate free CuNC and make it emit a bright and stable red fluorescence. A slight excess of Al in CuNC-Al solution can form a CTC-Al complex to limit the conformational rotation of CTC molecule and enhance CTC fluorescence. So, the red fluorescence of CuNC-Al probe and the enhanced CTC fluorescence are used as a reference signal and a response signal to detect CTC, respectively. The method developed shows a good linear relationship between the CTC concentration and the fluorescence intensity ratio (I/I) in the range 0.1-3.0 µM, and the detection limit is 25.3 nM (S/N = 3). In addition, the fluorescent color of CuNC-Al probe changes from red to yellow-green as the concentration of CTC increases. Based on this observation, a fluorescent test paper has also been fabricated. Schematic illustration of Al inducing CuNC to produce AIE performance and detecting CTC.
铜纳米团簇（CuNC）的聚集诱导发光（AIE）特性首次被用于构建比率荧光探针（CuNC-Al），以检测金霉素（CTC）。铝离子（Al）可以聚集游离的 CuNC，使其发出明亮而稳定的红色荧光。在 CuNC-Al 溶液中稍微过量的 Al 可以形成 CTC-Al 配合物，限制 CTC 分子的构象旋转并增强 CTC 荧光。因此，CuNC-Al 探针的红色荧光和增强的 CTC 荧光分别用作检测 CTC 的参考信号和响应信号。所开发的方法在 0.1-3.0 μM 范围内，CTC 浓度与荧光强度比（I/I）之间呈现出良好的线性关系，检测限为 25.3 nM（S/N=3）。此外，随着 CTC 浓度的增加，CuNC-Al 探针的荧光颜色从红色变为黄绿光。基于这一观察，还制备了荧光试纸。Al 诱导 CuNC 产生 AIE 性能并检测 CTC 的示意图。
