Baioumy Shereen A, Fouad Shaimaa H, Abdalgeleel Shaimaa A, Baiomy Ahmed A, Sallam Dina E, Taha Sara I
Department of Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Department of Internal Medicine / Allergy and Clinical Immunology, 68792Ain Shams University, Cairo, Egypt.
Int J Immunopathol Pharmacol. 2021 Jan-Dec;35:20587384211064454. doi: 10.1177/20587384211064454.
Dysregulation of the immune response appears to play a significant role in recurrent aphthous stomatitis (RAS) development. The main objective of this case-control study is to investigate the blood levels of mannose-binding lectin (MBL) and the frequency of the MBL2 gene (gly54asp) polymorphism in RAS patients, including 40 RAS patients and 40 healthy controls. Serum MBL levels were determined by ELISA, while the PCR-restriction fragment length polymorphism was used in MBL2 genotyping. The median serum MBL level was significantly lower in the RAS group than in the control group (975 ng/mL (545-1320) vs. 1760 ng/mL (1254-2134); ≤ 0.001). The MBL levels were significantly lower in the BB genotype, whereas they were significantly higher in the wild type AA with a median of 525 and 1340 ng/mL, respectively ( =0.005). The B allele was expressed in significantly higher percentages of RAS patients than in controls. There was no significant association between MBL serum levels (=0.685) or MBL2 codon 54 genotypes (=0.382) with the type of ulcers. There was an association between low MBL serum levels and the variant allele B of the MBL2 (gly54asp) gene, and the susceptibility to RAS. As a result, potential novel therapeutic options for RAS patients with MBL deficiency should be investigated.
免疫反应失调似乎在复发性阿弗他口炎（RAS）的发展中起重要作用。本病例对照研究的主要目的是调查RAS患者中甘露糖结合凝集素（MBL）的血液水平以及MBL2基因（gly54asp）多态性的频率，其中包括40例RAS患者和40例健康对照。通过酶联免疫吸附测定法（ELISA）测定血清MBL水平，而聚合酶链反应-限制性片段长度多态性用于MBL2基因分型。RAS组的血清MBL水平中位数显著低于对照组（975 ng/mL（545 - 1320）对1760 ng/mL（1254 - 2134）；≤0.001）。BB基因型的MBL水平显著较低，而野生型AA的MBL水平显著较高，中位数分别为525和1340 ng/mL（=0.005）。RAS患者中B等位基因的表达百分比显著高于对照组。MBL血清水平（=0.685）或MBL2密码子54基因型（=0.382）与溃疡类型之间无显著关联。MBL血清水平低与MBL2（gly54asp）基因的变异等位基因B以及RAS易感性之间存在关联。因此，应研究针对MBL缺乏的RAS患者的潜在新型治疗选择。
