Department of Clinical Microbiology, Aarhus Universitygrid.7048.b Hospital, Aarhus, Denmark.
Department of Dentistry and Oral Health, Aarhus Universitygrid.7048.b, Aarhus, Denmark.
Microbiol Spectr. 2021 Dec 22;9(3):e0108521. doi: 10.1128/Spectrum.01085-21. Epub 2021 Dec 15.
We developed a multilocus sequence typing scheme (MLST) for Aggregatibacter actinomycetemcomitans based on seven housekeeping genes, , , , , , , and . A total of 188 strains of seven serotypes were separated into 57 sequence types. Whole-genome sequences were available for 140 strains, and in contrast to comparison of 16S rRNA genes, phylogenetic analysis of concatenated MLST gene fragments was in accordance with the population structure revealed by alignment of 785 core genes. MLST could not decisively identify the so-called JP2 clone associated with rapidly progressing periodontitis in adolescents, but noticeable clustering of JP2 genotype strains was revealed. The MLST scheme of A. actinomycetemcomitans can be assessed at www.pubmlst.org. Accurate diagnosis of infectious disease comprise identification, typing, and antimicrobial resistance of the infective agent. Bacteria are sometimes grouped within their species according to expression of specific toxins or particular antimicrobial resistance traits, but explicit typing for infection control and survey of pathogenesis necessitates genetic analysis such as multilocus sequence typing (MLST). Schemes for the most prevalent human pathogens have been available for more than 10 years, and time has come to extend the scrutiny to second-line infectious agents. One such pathogen is , which is commonly involved in periodontitis, and more rarely as the cause of infective endocarditis or spontaneous brain abscess. A MLST scheme for is now available at www.pubmlst.org. Whole-genome sequencing of a large number of isolates confirms that MLST competently depicts the population structure of the species.
我们基于 7 个管家基因（,,,,,, 和 ）为伴放线放线杆菌（Aggregatibacter actinomycetemcomitans）开发了一种多位点序列分型方案（MLST）。7 种血清型的 188 株菌株被分为 57 种序列型。全基因组序列可用于 140 株菌株，与 16S rRNA 基因的比较相反，串联 MLST 基因片段的系统发育分析与通过 785 个核心基因比对揭示的种群结构一致。MLST 不能明确识别与青少年快速进展性牙周炎相关的所谓 JP2 克隆，但明显显示了 JP2 基因型菌株的聚类。A. actinomycetemcomitans 的 MLST 方案可在 www.pubmlst.org 进行评估。
传染病的准确诊断包括感染因子的鉴定、分型和抗菌药物耐药性。根据特定毒素或特定抗菌药物耐药性特征的表达，细菌有时会在其种内分组，但为了感染控制和发病机制调查，明确的分型需要进行遗传分析，如多位点序列分型（MLST）。最常见的人类病原体的方案已经有 10 多年的历史了，现在是时候将审查范围扩大到二线感染病原体了。其中一种病原体是 ，它通常与牙周炎有关，更罕见的是引起感染性心内膜炎或自发性脑脓肿。现在可以在 www.pubmlst.org 获得 的 MLST 方案。大量分离株的全基因组测序证实，MLST 能够很好地描述该物种的种群结构。
