Trevillyan J M, Canna C, Maley D, Linna T J, Phillips C A
Biochem Biophys Res Commun. 1986 Oct 15;140(1):392-8. doi: 10.1016/0006-291x(86)91103-4.
We have recently cloned a cDNA from the human T-cell leukemia, JURKAT, having homology with the src-like family of protein-tyrosine kinases. We have made rabbit polyclonal antibodies against the synthetic peptide CKERPEDRPTFDYLRSVLEDFFTATEGQYQPQP (cys-33-pro) deduced from the carboxy-terminal amino acid sequence predicted by the JURKAT cDNA. In this report, we demonstrate that these antibodies immunoprecipitate the protein-tyrosine kinase activity from solubilized membrane extracts from JURKAT T-leukemia cells and from human peripheral blood T-lymphocytes from normal donors. A 58 kd protein, exhibiting protein-tyrosine kinase activity, was specifically immunoprecipitated in both cases. The antibodies failed to crossreact with pp60c-src from human platelets, but did crossreact with the murine T-lymphocyte protein-tyrosine kinase, pp56T-cell.
我们最近从人T细胞白血病JURKAT中克隆了一个与src样蛋白酪氨酸激酶家族具有同源性的cDNA。我们针对由JURKAT cDNA预测的羧基末端氨基酸序列推导的合成肽CKERPEDRPTFDYLRSVLEDFFTATEGQYQPQP（cys-33-pro）制备了兔多克隆抗体。在本报告中，我们证明这些抗体能从JURKAT T白血病细胞和正常供体的人外周血T淋巴细胞的可溶性膜提取物中免疫沉淀蛋白酪氨酸激酶活性。在这两种情况下，均特异性免疫沉淀出一种具有蛋白酪氨酸激酶活性的58 kd蛋白。这些抗体未能与人血小板中的pp60c-src发生交叉反应，但与鼠T淋巴细胞蛋白酪氨酸激酶pp56T-cell发生了交叉反应。
