Trevillyan J M, Lin Y, Chen S J, Phillips C A, Canna C, Linna T J
Biochim Biophys Acta. 1986 Oct 10;888(3):286-95. doi: 10.1016/0167-4889(86)90228-4.
We have used oligonucleotide probes, based on a portion of the p60v-src autophosphorylation sequence, Glu-Asp-Asn-Glu-Tyr-Thr, to identify and characterize a cDNA from the human T-leukemia cell line, JURKAT. The JURKAT cDNA (designated ptk-JURKAT) was homologous to but distinct from the src, yes and fgr oncogenes, which encode protein-tyrosine kinases (ATP:protein phosphotransferase, EC 2.7.1.37). The ptk-JURKAT cDNA hybridized with a 2.2 kb RNA transcript from JURKAT cells and the human T-cell lymphoma line, MOLT-4, but failed to identify any transcript in two human B-cell lymphoma lines or a human erythroid-myeloid leukemia line, K562. Recently the nucleotide sequence has been established for the murine lymphocyte protein tyrosine kinase, p56LSTRA. The ptk-JURKAT cDNA appears to encode the human homolog of p56LSTRA.
我们使用了基于p60v-src自身磷酸化序列（Glu-Asp-Asn-Glu-Tyr-Thr）一部分的寡核苷酸探针，来鉴定和表征来自人T淋巴细胞白血病细胞系JURKAT的一个cDNA。JURKAT cDNA（命名为ptk-JURKAT）与src、yes和fgr癌基因同源，但又有所不同，这些癌基因编码蛋白质酪氨酸激酶（ATP：蛋白质磷酸转移酶，EC 2.7.1.37）。ptk-JURKAT cDNA与来自JURKAT细胞和人T细胞淋巴瘤系MOLT-4的一个2.2 kb RNA转录本杂交，但未能在两个人B细胞淋巴瘤系或人红系-髓系白血病系K562中鉴定出任何转录本。最近已经确定了鼠淋巴细胞蛋白质酪氨酸激酶p56LSTRA的核苷酸序列。ptk-JURKAT cDNA似乎编码p56LSTRA的人同源物。
