Park H
J Immunol Methods. 1978;20:349-55. doi: 10.1016/0022-1759(78)90270-3.
A new, simple, inexpensive plastic device for solid phase immunoassay, the through-passage receptacle (TPR), with a large ratio of surface area to volume, is described. It was substantially more efficient in non-specific protein adsorption and specific immunological binding than the conventional wells of microtiter plates. It was also easier to wash since washing was facilitated by a through-passage with a gravity flow. This device, which should be more conducive to automation than currently used receptacles, should increase the sensitivity and decrease the incubation time of the solid phase immunoassay technique.
本文描述了一种新型、简单、廉价的用于固相免疫测定的塑料装置——直通式容器（TPR），其表面积与体积之比很大。在非特异性蛋白质吸附和特异性免疫结合方面，它比传统的微量滴定板孔更高效。由于有重力流的直通通道便于洗涤，所以它也更容易清洗。这种装置应该比目前使用的容器更有利于自动化，应能提高固相免疫测定技术的灵敏度并缩短孵育时间。
