Park H
Clin Chem. 1979 Jan;25(1):178-82.
I describe a new technique for improving the sensitivity of the solid-phase "sandwich" assay, by using the through-passage receptacle in a novel flow-communication device. The technique allows a large volume of serum to flow through the antibody-coated receptacle repeatedly during the incubation and is thus termed the "flow-through large-volume incubation" method. Binding of 125I-labeled hepatitis B surface antigen to its corresponding antibody on a solid-phase by this method was more rapid and persistent than binding by the conventional method. When the method was applied to the first incubation of the sandwich assay, the test for the antigen was rendered four-, eight-, and 32-fold as sensitive as an accepted third-generation test for the antigen, by incubating 5-mL volumes of serum at (a) room temperature for 18 h, (b) 45 degrees C for 8 h, or (c) room temperature for seven days, respectively.
我描述了一种通过在新型流动连通装置中使用直通式容器来提高固相“夹心”检测灵敏度的新技术。该技术可使大量血清在孵育期间反复流经包被抗体的容器，因此被称为“流通式大容量孵育”方法。通过这种方法，125I标记的乙型肝炎表面抗原与固相上相应抗体的结合比传统方法更快速且持久。当该方法应用于夹心检测的首次孵育时，通过分别在（a）室温下孵育5毫升血清18小时、（b）45℃孵育8小时或（c）室温下孵育7天，对抗原的检测灵敏度比公认的第三代抗原检测高4倍、8倍和32倍。
