从小鼠中分离CD31骨髓内皮细胞（BMECs）。
Isolation of CD31 Bone Marrow Endothelial Cells (BMECs) from Mice.
作者信息
Smith Alhaji Osman, Adzraku Seyram Yao, Ju Wen, Qiao Jianlin, Xu Kailin, Zeng Lingyu
机构信息
Blood Diseases Institute, Xuzhou Medical University, Xuzhou 221002, China.
Key Laboratory of the Bone Marrow Stem Cell Transplantation, Xuzhou 221002, China.
出版信息
Bio Protoc. 2021 Nov 20;11(22):e4227. doi: 10.21769/BioProtoc.4227.
In the bone marrow microenvironment, endothelial cells (ECs) play a pivotal role in regulating the production of both growth and inhibiting factors. They are held together by adherence molecules that interact with hematopoietic progenitor cells. The study of ECs in the hematopoietic stem cell niche is limited due to the lack of efficient protocols for isolation. In this protocol, we developed a two-step approach to extract bone marrow endothelial cells (BMECs) to unlock the challenges researchers face in understanding the function of the endothelial vascular niche in studies.
在骨髓微环境中，内皮细胞（ECs）在调节生长因子和抑制因子的产生方面发挥着关键作用。它们通过与造血祖细胞相互作用的黏附分子聚集在一起。由于缺乏有效的分离方案，造血干细胞龛中内皮细胞的研究受到限制。在本方案中，我们开发了一种两步法来提取骨髓内皮细胞（BMECs），以解决研究人员在理解内皮血管龛功能时面临的挑战。
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