López-Marqués Rosa L, Pomorski Thomas G
Department of Plant and Environmental Sciences, Copenhagen Plant Science Center, University of Copenhagen, Frederiksberg C, Denmark.
Department of Molecular Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr University Bochum, Bochum, Germany.
Bio Protoc. 2021 Nov 20;11(22):e4228. doi: 10.21769/BioProtoc.4228.
Eukaryotic cells use a diverse set of transporters to control the movement of lipids across their plasma membrane, which drastically affects membrane properties. Various tools and techniques to analyze the activity of these transporters have been developed. Among them, assays based on fluorescent phospholipid probes are particularly suitable, allowing for imaging and quantification of lipid internalization in living cells. Classically, these assays have been applied to yeast and animal cells. Here, we describe the adaptation of this powerful approach to characterize lipid internalization in plant roots and aerial tissues using confocal imaging. Graphic abstract: Scale bars: seedling, 25 mm; leaf, 10 μm; root, 25 μm.
真核细胞利用多种转运蛋白来控制脂质跨质膜的移动，这会极大地影响膜的特性。人们已经开发出各种分析这些转运蛋白活性的工具和技术。其中，基于荧光磷脂探针的检测方法特别适用，可对活细胞中的脂质内化进行成像和定量分析。传统上，这些检测方法已应用于酵母和动物细胞。在此，我们描述了如何采用这种强大的方法，利用共聚焦成像来表征植物根和地上组织中的脂质内化。图形摘要：比例尺：幼苗，25毫米；叶片，10微米；根，25微米。
