Gray P W, Glaister D, Chen E, Goeddel D V, Pennica D
J Immunol. 1986 Dec 1;137(11):3644-8.
A human interleukin 1 beta (IL 1 beta) cDNA probe was utilized to identify a homologous murine cDNA clone. The murine cDNA encodes a 269-residue protein which is 67% homologous to human IL 1 beta. The murine sequence was engineered for expression in mammalian cells and directs the synthesis of biologically active IL 1. This protein, termed murine IL 1 beta, is only 22% homologous with the previously described murine IL 1 sequence. Both IL 1 alpha and IL 1 beta are encoded by single genes, but IL 1 beta mRNA is about fivefold more abundant in a stimulated macrophage cell line.
使用人白细胞介素1β（IL - 1β）cDNA探针来鉴定同源的小鼠cDNA克隆。该小鼠cDNA编码一种由269个氨基酸残基组成的蛋白质，它与人IL - 1β的同源性为67%。对小鼠序列进行工程改造以在哺乳动物细胞中表达，并指导合成具有生物活性的IL - 1。这种蛋白质被称为小鼠IL - 1β，它与先前描述的小鼠IL - 1序列的同源性仅为22%。IL - 1α和IL - 1β均由单基因编码，但在受刺激的巨噬细胞系中，IL - 1β mRNA的丰度约高五倍。
