Howard R D, McIlwraith C W, Trotter G W, Nyborg J K
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523, USA.
Am J Vet Res. 1998 Jun;59(6):704-11.
To clone equine interleukin 1 alpha (IL-1 alpha) and equine interleukin 1 beta (IL-1 beta) and determine their full-length cDNA sequences.
The mRNA isolated from lipopolysaccharide-stimulated cultured equine monocytes was reverse transcribed, and a cDNA library was constructed in a lambda phage. The cDNA library was screened by means of plaque hybridization with radiolabeled human IL-1 alpha and IL-1 beta cDNA probes. The cDNA nucleotide sequences for equine IL-1 alpha and equine IL-1 beta were determined by use of the dideoxy chain termination technique. The cDNA sequences were analyzed, using computer software, for sequence characteristics and compared with sequences reported for other species.
The cDNA for equine IL-1 alpha was 1,728 base pairs in length with an ORF encoding a peptide of 270 amino acids with a predicted molecular mass of 30.823 kd. The cDNA for equine IL-1 beta was 1,473 base pairs in length with an ORF encoding a peptide of 268 amino acids with a predicted molecular mass of 30.342 kd. Similarity between amino acid sequence of equine IL-1 alpha and sequences for IL-1 alpha of other species ranged from 62.5 to 82.2%; similarity between amino acid sequence of equine IL-1 beta and sequences for IL-1 beta of other species ranged from 62.5 to 82.2%; similarity between amino acid sequences of equine IL-1 alpha and equine IL-1 beta was 26%.
Results establish a basis for studying the roles of interleukin 1 in healthy and diseased joints in horses.
克隆马白细胞介素1α（IL-1α）和马白细胞介素1β（IL-1β），并确定它们的全长cDNA序列。
从脂多糖刺激培养的马单核细胞中分离出mRNA进行逆转录，并构建λ噬菌体cDNA文库。用放射性标记的人IL-1α和IL-1β cDNA探针通过噬菌斑杂交筛选该cDNA文库。采用双脱氧链终止技术测定马IL-1α和马IL-1β的cDNA核苷酸序列。利用计算机软件分析cDNA序列的特征，并与其他物种报道的序列进行比较。
马IL-1α的cDNA长度为1728个碱基对，开放阅读框编码一个270个氨基酸的肽，预测分子量为30.823kd。马IL-1β的cDNA长度为1473个碱基对，开放阅读框编码一个268个氨基酸的肽，预测分子量为30.342kd。马IL-1α氨基酸序列与其他物种IL-1α序列的相似性在62.5%至82.2%之间；马IL-1β氨基酸序列与其他物种IL-1β序列的相似性在62.5%至82.2%之间；马IL-1α和马IL-1β氨基酸序列的相似性为26%。
研究结果为研究白细胞介素1在马健康和患病关节中的作用奠定了基础。
