Gascan H, Moreau J F, Jacques Y, Soulillou J P
INSERM U.211, Faculté de Médecine, Nantes, France.
Lymphokine Res. 1989 Spring;8(1):79-84.
We analyzed the proliferative response of DA1, DA1-a, NFS 60, 32DC1, Ea 3-17 and FDCP2 murine interleukin 3 (mIL3)-sensitive cell lines to human recombinant IL1 alpha, IL1 beta, IL2, IL4, IL5, IL6, granulocyte-CSF (G-CSF), macrophage-CSF (M-CSF), granulocyte-macrophage-CSF (GM-CSF), interferon gamma (IFN gamma), tumor necrosis factor (TNF) alpha, gibbon IL3, purified HILDA and mouse recombinant IL3, IL4, GM-CSF. All cell lines responded to mIL3 and IL4. Various response patterns were observed with human IL2, G-CSF and murine GM-CSF. Human IL4 and GM-CSF were absolutely inefficient in triggering proliferation of lines sensitive to their murine counterparts, confirming the species specificity of these two cytokines. Among the cell lines responding to G-CSF, some variations in sensitivities were observed. Thus the 32DC1 cell line needed 10 to 30 times more G-CSF to reach the same level of proliferation as the NFS60 cell line. DA1-a was the only factor-dependent cell line responding to purified HILDA, and IL6 was found to trigger proliferation of the NFS60 cell line, with a half-maximum effect observed for 0.1 pM of hormone.
我们分析了DA1、DA1-a、NFS 60、32DC1、Ea 3-17和FDCP2小鼠白细胞介素3（mIL3）敏感细胞系对人重组IL1α、IL1β、IL2、IL4、IL5、IL6、粒细胞集落刺激因子（G-CSF）、巨噬细胞集落刺激因子（M-CSF）、粒细胞巨噬细胞集落刺激因子（GM-CSF）、干扰素γ（IFNγ）、肿瘤坏死因子（TNF）α、长臂猿IL3、纯化的HILDA以及小鼠重组IL3、IL4、GM-CSF的增殖反应。所有细胞系均对mIL3和IL4有反应。用人IL2、G-CSF和小鼠GM-CSF观察到了不同的反应模式。人IL4和GM-CSF在触发对其小鼠对应物敏感的细胞系增殖方面绝对无效，证实了这两种细胞因子的种属特异性。在对G-CSF有反应的细胞系中，观察到了敏感性的一些差异。因此，32DC1细胞系达到与NFS60细胞系相同增殖水平所需的G-CSF要多10至30倍。DA1-a是唯一对纯化的HILDA有反应的因子依赖性细胞系，并且发现IL6可触发NFS60细胞系的增殖，0.1 pM的激素可观察到半数最大效应。
