Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.
Student Research Committee, Faculty of Allied Medicine, Kerman University of Medical Sciences, Kerman, Iran.
J Integr Bioinform. 2021 Dec 16;19(1):20210016. doi: 10.1515/jib-2021-0016.
Aberrant expression of genes involved in methylation, including DNA methyltransferase 3 Beta (), can cause hypermethylation of various tumor suppressor genes. In this regard, various molecular factors such as microRNAs can play a critical role in regulating these methyltransferase enzymes and eventually downstream genes such as growth arrest specific 7 (). Accordingly, in the present study we aimed to predict regulatory effect of miRNAs on and genes expression in melanoma cell line. hsa-miR-203a-3p and hsa-miR-29a-3p were predicted and selected using bioinformatics software. The Real-time PCR technique was performed to investigate the regulatory effect of these molecules on the and genes expression. Expression analysis of gene in A375 cell line showed that there was a significant increase compared to control ( value = 0.0015). Analysis of hsa-miR-203a-3p and hsa-miR-29a-3p indicated the insignificant decreased expression in melanoma cell line compared to control ( value < 0.05). Compared to control, the expression of GAS7 gene in melanoma cells showed a significant decrease ( value = 0.0323). Finally, our findings showed that the decreased expression of hsa-miR-203a-3p and hsa-miR-29a-3p can hypothesize that their aberrant expression caused dysfunction, possible methylation of the gene, and ultimately decreased its expression. However, complementary studies are necessary to definite comment.
参与甲基化的基因异常表达，包括 DNA 甲基转移酶 3β ()，可导致各种肿瘤抑制基因的过度甲基化。在这方面，各种分子因素，如 microRNAs，可以在调节这些甲基转移酶酶和最终的下游基因，如生长停滞特异性 7（）中发挥关键作用。因此，在本研究中，我们旨在预测 microRNAs 对黑色素瘤细胞系中基因和基因表达的调控作用。使用生物信息学软件预测和选择 hsa-miR-203a-3p 和 hsa-miR-29a-3p。采用实时 PCR 技术研究这些分子对基因和基因表达的调控作用。基因在 A375 细胞系中的表达分析表明，与对照组相比，表达显著增加（值=0.0015）。hsa-miR-203a-3p 和 hsa-miR-29a-3p 的分析表明，与对照组相比，黑色素瘤细胞系中的表达显著降低（值<0.05）。与对照组相比，黑色素瘤细胞中 GAS7 基因的表达显著降低（值=0.0323）。最后，我们的研究结果表明，hsa-miR-203a-3p 和 hsa-miR-29a-3p 的表达降低可能假设其异常表达导致功能障碍，可能导致基因甲基化，最终导致其表达降低。然而，还需要进一步的研究来明确这一点。
