Yamada M, Matsumoto Y, Hamada S, Fujita S, Yoshida Y
Zentralbl Bakteriol Mikrobiol Hyg A. 1986 Aug;262(2):240-6. doi: 10.1016/s0176-6724(86)80025-6.
We have attempted to demonstrate DNA and to measure the amount of nuclear DNA in Pneumocystis carinii by fluorescence microscopy with 4', 6-diamidino-2-phenylindole (DAPI). Due to the high specificity of DAPI for DNA, only nuclei and mitochondria were stained. An uninucleate trophozoite and a mature cyst containing eight intracystic bodies were distinguished easily each other after staining with DAPI. The total amount of nuclear DNA of eight intracystic bodies in a cyst was approximately eight times that of the small type of trophozoite. When nuclei of uninucleate trophozoites were randomly examined, the distribution pattern of nuclear fluorescence intensity showed two peaks, one at 1C and the other at 2C. Moreover, it showed also a gradual transition in DNA values from 1C to 2C, and very few nuclei with polyploid DNA values. The primary advantage of this method with DAPI is that it allows a more efficient evaluation of the life cycle of P. carinii.
我们尝试通过使用4',6-二脒基-2-苯基吲哚（DAPI）的荧光显微镜来显示卡氏肺孢子虫的DNA并测量其核DNA的量。由于DAPI对DNA具有高度特异性，因此仅细胞核和线粒体被染色。在用DAPI染色后，很容易区分出单核滋养体和含有八个囊内小体的成熟包囊。一个包囊内八个囊内小体的核DNA总量约为小型滋养体的八倍。当随机检查单核滋养体的细胞核时，核荧光强度的分布模式显示出两个峰值，一个在1C，另一个在2C。此外，它还显示出DNA值从1C到2C的逐渐转变，并且具有多倍体DNA值的细胞核非常少。这种使用DAPI的方法的主要优点是它可以更有效地评估卡氏肺孢子虫的生命周期。
