Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Int J Biol Macromol. 2022 Jan 15;195:255-263. doi: 10.1016/j.ijbiomac.2021.12.019. Epub 2021 Dec 14.
Several species of novel marine bacteria from the genus Marinobacterium, including M. nitratireducens, M. sediminicola, and M. zhoushanense were found to be capable of producing polyhydroxyalkanoates (PHA) using sugars and volatile fatty acids (VFAs) as the carbon source. M. zhoushanense produced poly-3-hydroxybutytate (PHB) from sucrose, achieving a product titer and PHB content of 2.89 g/L and 64.05 wt%, respectively. By contrast, M. nitratireducens accumulated 3.38 g/L PHB and 66.80 wt% polymer content using butyrate as the substrate. A third species, M. sediminicola showed favorable tolerance to propionate, butyrate, and valerate. The use of 10 g/L valerate yielded 3.37 g/L poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), with a 3-hydroxyvalerate (3 HV) monomer content of 94.75 mol%. Moreover, M. sediminicola could be manipulated to produce PHBV with changeable polymer compositions by feeding different mixtures of VFAs. Our results indicate that M. sediminicola is a promising halophilic bacterium for the production of PHA.
几种新型海洋细菌 Marinobacterium 属，包括 Marinobacterium nitratireducens、Marinobacterium sediminicola 和 Marinobacterium zhoushanense，被发现能够使用糖和挥发性脂肪酸 (VFA) 作为碳源生产聚羟基烷酸酯 (PHA)。Marinobacterium zhoushanense 从蔗糖中生产聚-3-羟基丁酸酯 (PHB)，达到 2.89 g/L 的产物浓度和 64.05 wt%的 PHB 含量。相比之下，Marinobacterium nitratireducens 使用丁酸盐作为底物积累了 3.38 g/L PHB 和 66.80 wt%聚合物含量。第三种，Marinobacterium sediminicola 对丙酸、丁酸和戊酸表现出良好的耐受性。使用 10 g/L 戊酸可得到 3.37 g/L 的聚（3-羟基丁酸酯-共-3-羟基戊酸酯）（PHBV），其中 3-羟基戊酸（3-HV）单体含量为 94.75 mol%。此外，通过喂养不同的 VFA 混合物，可以操纵 Marinobacterium sediminicola 生产具有可变聚合物组成的 PHBV。我们的结果表明，Marinobacterium sediminicola 是一种有前途的生产 PHA 的嗜盐细菌。
