Kim Jeongtae, Ahn Meejung, Choi Yuna, Chun Jiyoon, Jung Kyungsook, Tanaka Akane, Matsuda Hiroshi, Shin Taekyun
Department of Anatomy, Kosin University College of Medicine, Busan, Republic of Korea.
Department of Animal Science, College of Life Science, Sangji University, Wonju, Republic of Korea.
Neural Regen Res. 2022 Jul;17(7):1604-1608. doi: 10.4103/1673-5374.330614.
Osteopontin (OPN) is an extracellular matrix protein with a diverse range of functions, including roles in cell adhesion, migration, and immunomodulation, which are associated with the modulation of neuroinflammation in the central nervous system. The present study was performed to evaluate the involvement of OPN in the eyes of an experimental autoimmune uveoretinitis (EAU) model. The EAU model was developed by immunization of Lewis rats with interphotoreceptor retinoid-binding protein. The results showed the OPN level was remarkably upregulated in the eye of EAU rats on day 9 post-immunization. The level of CD44, a ligand of OPN, was increased in the ciliary body of EAU rats. Furthermore, OPN was also detected in the ciliary body and activated microglia/macrophages in the EAU retina. The results suggest that OPN was significantly upregulated in the eyes of EAU rats, and that it may be useful as an early biomarker of ocular autoimmune diseases. All animal experiments were approved by the Institutional Animal Care and Use Committee of Jeju National University (approval No. 2020-0012) on March 11, 2020.
骨桥蛋白（OPN）是一种细胞外基质蛋白，具有多种功能，包括在细胞黏附、迁移和免疫调节中发挥作用，这些功能与中枢神经系统神经炎症的调节相关。本研究旨在评估OPN在实验性自身免疫性葡萄膜视网膜炎（EAU）模型眼中的作用。通过用视网膜色素上皮结合蛋白免疫Lewis大鼠建立EAU模型。结果显示，免疫后第9天，EAU大鼠眼中的OPN水平显著上调。OPN的配体CD44在EAU大鼠的睫状体中水平升高。此外，在EAU视网膜的睫状体和活化的小胶质细胞/巨噬细胞中也检测到了OPN。结果表明，EAU大鼠眼中的OPN显著上调，它可能作为眼部自身免疫性疾病的早期生物标志物。所有动物实验均于2020年3月11日获得济州国立大学机构动物护理和使用委员会批准（批准号：2020-0012）。
