New geographic records for in coyotes and moose from Nova Scotia, Canada.

spp. tapeworms can cause serious diseases in mammals, including humans. Within the species complex, metacestodes produce unilocular cysts that are responsible for cystic echinococcosis in animal intermediate hosts. Canids are definitive hosts, harbouring adult cestodes in their intestines. Adult were recovered from the small intestine of 1 of 262 coyotes () from Nova Scotia, Canada. Subsequently, we found unilocular cysts in lungs and livers of 4 of 8 sympatric moose () from Cape Breton Island. DNA was extracted from three cysts using the Qiagen DNeasy Blood and Tissue kit and assayed by polymerase chain reaction (PCR) with primers (cest4 and cest5) for a 117-bp region of the small subunit of ribosomal RNA of , and further validated as G8 using primers targeting nicotinamide adenosine dinucleotide dehydrogenase subunit 1 (ND1) and cytochrome oxidase subunit 1 (CO1) mitochondrial genes. These are the first records of in any of the three Maritime provinces, which include Nova Scotia, New Brunswick, and Prince Edward Island. The parasite was thought to be absent in this region due to extirpation of wolves ( spp.) in the 1800s. These findings suggest that further wildlife surveillance and risk assessment is warranted.