Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Juhan Liivi 2, 50409 Tartu, Estonia.
Estonian Veterinary and Food Laboratory, Kreutzwaldi 30, 51006 Tartu, Estonia.
Parasitology. 2023 Jun;150(7):631-637. doi: 10.1017/S0031182023000331. Epub 2023 Apr 3.
is a group of tapeworm species known to cause cystic echinococcosis. Within this group, the cluster includes genotypes G8 and G10 that have a predominantly sylvatic life cycle – transmission occurs between wild cervids and wolves. Relatively few studies have explored the genetic variation of the elusive G8 and G10, and their extent of genetic variation is yet to be investigated at the complete mitochondrial (mt) genome level. The aim was to explore the genetic variation of these 2 genotypes in Europe using complete mtDNA sequences and provide a high-quality reference dataset for future studies. Sequences of complete mt genomes were produced for 29 samples of genotype G8 and G10 from wolves, moose, reindeer and roe deer, originating from Finland, Sweden, Russia, Poland, Latvia and Estonia. Genetic variation was explored based on phylogenetic network analysis, revealing marked differences between G8 and G10 (over 400 mutations), and more detailed patterns of variability within the 2 genotypes than previously observed. Understanding the mt genetic composition of a species provides a baseline for future studies aiming to understand whether this mt distinctiveness is mirrored in the nuclear genome and whether it has any impact on any phenotypic traits or parasite transmission.
是一组已知会引起包虫病的绦虫物种。在这一组中，簇包括基因型 G8 和 G10，它们主要具有森林生境生命周期——在野生鹿科动物和狼之间传播。相对较少的研究探索了难以捉摸的 G8 和 G10 的遗传变异，它们的遗传变异程度尚未在完整的线粒体（mt）基因组水平上进行研究。本研究旨在使用完整的 mtDNA 序列探索欧洲这两种基因型的遗传变异，并为未来的研究提供高质量的参考数据集。对来自芬兰、瑞典、俄罗斯、波兰、拉脱维亚和爱沙尼亚的狼、驼鹿、驯鹿和狍子的 29 个 G8 和 G10 基因型样本的完整 mt 基因组序列进行了测序。基于系统发育网络分析探讨了遗传变异，揭示了 G8 和 G10 之间的显著差异（超过 400 个突变），并且在这两种基因型内的变异性模式比以前观察到的更为详细。了解物种的 mt 遗传组成为未来的研究提供了一个基线，旨在了解这种 mt 独特性是否在核基因组中得到反映，以及它是否对任何表型特征或寄生虫传播有任何影响。
