Penner E
Gastroenterology. 1987 Feb;92(2):304-8. doi: 10.1016/0016-5085(87)90122-3.
Immune complexes containing antinuclear antibodies have been observed in autoimmune chronic active hepatitis. Using a library of nuclear constituents (deoxyribonucleic acid, small nuclear ribonucleoproteins, and histones), we analyzed the antigens involved in formation of immune complexes. Immune complexes were defined in dissociation experiments, and after separation under dissociating conditions. Immune complexes composed of histones and immunoglobulin G were observed in four of nine immune complex-positive autoimmune chronic active hepatitis sera. Immune complexes containing the small nuclear ribonucleoprotein U1-RNP and immunoglobulin G were observed in two additional samples. Kidney eluates obtained from 2 patients with autoimmune chronic active hepatitis and membranous glomerulonephritis revealed enrichment of anti-U1-RNP, suggesting specific deposition of this antibody in complexed form. Circulating immune complexes containing histones were observed only in patients with autoimmune chronic active hepatitis-associated sicca syndrome; those containing U1-RNP were restricted to patients with autoimmune chronic active hepatitis associated with kidney disease.
在自身免疫性慢性活动性肝炎中已观察到含有抗核抗体的免疫复合物。利用一个包含核成分（脱氧核糖核酸、小核核糖核蛋白和组蛋白）的文库，我们分析了参与免疫复合物形成的抗原。免疫复合物在解离实验中以及在解离条件下分离后被定义。在9份免疫复合物阳性的自身免疫性慢性活动性肝炎血清中的4份中观察到由组蛋白和免疫球蛋白G组成的免疫复合物。在另外2份样本中观察到含有小核核糖核蛋白U1-RNP和免疫球蛋白G的免疫复合物。从2例自身免疫性慢性活动性肝炎合并膜性肾小球肾炎患者获得的肾洗脱液显示抗U1-RNP富集，提示该抗体以复合形式特异性沉积。仅在伴有干燥综合征的自身免疫性慢性活动性肝炎患者中观察到含有组蛋白的循环免疫复合物；那些含有U1-RNP的复合物仅限于与肾脏疾病相关的自身免疫性慢性活动性肝炎患者。
