Chida S, Sakamoto M, Takino T, Kawamoto S, Hagiwara K
School of veterinary Medicene, Rakuno Gakuen University, 582 Bunkyodai Ebetsu, Hokkaido, 069-8501 Japan.
Scientific Feed Laboratory co., ltd., R & D center, Sakura city, Chiba, 285-0043 Japan.
Vet Anim Sci. 2021 Dec 2;14:100222. doi: 10.1016/j.vas.2021.100222. eCollection 2021 Dec.
High-yield dairy cows need high energy feed during periods of increased milk production. The transitional feeding to high energy feed increases the risk of developing a variety of metabolic disorders. Here, five Holstein cows were fed a four-stage feeding protocol (3 weeks for each stage) ranging from 54.9 to 73.7% total digestive nutrients (TDN). The purpose of the study was to investigate the effect of lactic acid bacteria on high-energy-fed cows associated with transitional feeding, and to evaluate the effects of probiotics on intestinal bacterial changes and inflammatory responses. Three feed transition periods were established for five cows, and RGU-LP1 (LP1) was fed as a probiotic during the high-energy feeding period. The number of lymphocyte subsets such as CD3-, CD4-, and CD8 positive cells decreased in response to the high energy feed. Lipopolysaccharide (LPS)-induced cytokine ( and ) gene expression in peripheral blood mononuclear cells (PBMCs) was shown to increase in those animals receiving the high energy feed. However, supplementation with LP1 resulted in an increase in the number of lymphocyte subsets and the expression of and were returned to the level at low energy diet. These results suggest that high energy diets induce inflammatory cytokine responses following LPS stimulation, and that the addition of LP1 mitigates these results by regulating the LPS-induced inflammatory reaction. Therefore, the functional lactic acid bacteria LP1 is expected to regulate inflammation resulting from high energy feeding, and this probiotic could be applied to support inflammatory regulation in high-yield dairy cows.
高产奶牛在产奶量增加期间需要高能量饲料。向高能量饲料的过渡性喂养会增加患各种代谢紊乱疾病的风险。在此，五头荷斯坦奶牛按照四阶段喂养方案（每个阶段3周）进行喂养，总消化养分（TDN）范围从54.9%至73.7%。本研究的目的是调查乳酸菌对与过渡性喂养相关的高能量喂养奶牛的影响，并评估益生菌对肠道细菌变化和炎症反应的影响。为五头奶牛设定了三个饲料过渡期，在高能量喂养期间将RGU-LP1（LP1）作为益生菌投喂。响应高能量饲料，CD3、CD4和CD8阳性细胞等淋巴细胞亚群的数量减少。在接受高能量饲料的动物中，外周血单核细胞（PBMCs）中脂多糖（LPS）诱导的细胞因子（和）基因表达增加。然而，补充LP1导致淋巴细胞亚群数量增加，并且和的表达恢复到低能量饮食时的水平。这些结果表明，高能量饮食在LPS刺激后诱导炎症细胞因子反应，并且添加LP1通过调节LPS诱导的炎症反应减轻了这些结果。因此，功能性乳酸菌LP1有望调节高能量喂养引起的炎症，并且这种益生菌可用于支持高产奶牛的炎症调节。
