Department of Obstetrics and Gynecology/Section of Gynecologic Oncology, University of Chicago, Chicago, IL, USA.
Methods Mol Biol. 2022;2424:155-165. doi: 10.1007/978-1-0716-1956-8_10.
Cancer-associated fibroblasts (CAFs) play important roles in regulating tumor progression, metastasis, and response to therapies. Accurately modeling the interplay between cancer cells and the tumor microenvironment (TME) requires the use of primary cells from patient samples. Here we describe methods for the isolation of both primary CAFs and fibroblasts from omental tissue using a combination of mechanical dissociation and enzymatic digestion. Primary cells can be used for functional and mechanistic studies and may be safely cryopreserved.
癌症相关成纤维细胞（CAFs）在调节肿瘤进展、转移和对治疗的反应方面发挥着重要作用。准确地模拟癌细胞与肿瘤微环境（TME）之间的相互作用需要使用来自患者样本的原代细胞。在这里，我们描述了使用机械解离和酶消化相结合的方法从大网膜组织中分离原代 CAFs 和成纤维细胞的方法。原代细胞可用于功能和机制研究，并且可以安全地冷冻保存。
