Departamento de Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, MA 02114, USA; Department of Microbiology, Harvard Medical School, Boston, MA 02114, USA; Infectious Disease and Microbiome Program, Broad Institute, Cambridge, MA 02142, USA.
Infect Genet Evol. 2022 Jan;97:105189. doi: 10.1016/j.meegid.2021.105189. Epub 2021 Dec 14.
The surface protein SasX, has a key role in methicillin-resistant Staphylococcus aureus (MRSA) colonization and pathogenesis, and has been associated with the epidemic success of some MRSA clones. To date, only one SasX homologous protein, named SesI, has been described in Staphylococcus epidermidis. In this work, we analyze the occurrence of the sasX gene and its genetic environment in Staphylococcus haemolyticus S. haemolyticus clinical strains (n = 62) were screened for the presence of the sasX gene and its carrier, the prophage Φ SPβ-like. A deep characterization was done in one strain (MD43), through which we determined the complete nucleotide sequence for the S. haemolitycus sasX-like gene. Whole genome sequencing of strain MD43 was performed, and the gene, termed here because of its unique attributes, shsA, was mapped to the Φ SPβ-like prophage sequence. The shsA gene was detected in 33 out of 62 strains showing an average identity of 92 and 96% with the sasX and sesI genes and at the amino acid level, 88% identity with SasX and 92% identity with SesI. The ~124Kb Φ SPβ-like prophage sequence showed a largely intact prophage compared to its counterpart in S. epidermidis strain RP62A, including the sesI insertion site. In conclusion, we identified a new sasX ortholog in S. haemolyticus (shsA). Its horizontal spread from this reservoir could represent an emergent threat in healthcare facilities since so far, no S. aureus sasX strains have been reported in Brazil.
表面蛋白 SasX 在耐甲氧西林金黄色葡萄球菌（MRSA）定植和发病机制中起着关键作用，并且与一些 MRSA 克隆的流行成功有关。迄今为止，在表皮葡萄球菌中仅描述了一种 SasX 同源蛋白，称为 SesI。在这项工作中，我们分析了在 62 株化脓性链球菌临床菌株中 sasX 基因及其遗传环境的发生情况，筛选了 sasX 基因及其载体噬菌体 Φ SPβ 样的存在。对一株（MD43）进行了深入表征，通过该菌株确定了化脓性链球菌 sasX 样基因的完整核苷酸序列。对菌株 MD43 进行了全基因组测序，并将该基因命名为 shsA，因为其具有独特的属性，被映射到 Φ SPβ 样噬菌体序列上。在 62 株菌株中有 33 株检测到 shsA 基因，与 sasX 和 sesI 基因的平均同一性分别为 92%和 96%，在氨基酸水平上与 SasX 的同一性为 88%，与 SesI 的同一性为 92%。与表皮葡萄球菌菌株 RP62A 相比，~124Kb 的 Φ SPβ 样噬菌体序列显示出一个基本完整的噬菌体，包括 sesI 插入位点。总之，我们在化脓性链球菌中鉴定出一种新的 sasX 同源物（shsA）。由于迄今为止，巴西尚未报告过金黄色葡萄球菌 sasX 菌株，因此该基因从该储库中的水平传播可能代表医疗保健设施中的一个新出现的威胁。
