State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, China.
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, China.
Phytochemistry. 2022 Mar;195:113055. doi: 10.1016/j.phytochem.2021.113055. Epub 2021 Dec 14.
Two undescribed phenolic derivatives musaones A and B, six undescribed γ-butyrolactone musaolides A-F, together with two undescribed cyclopentanone musaolides G and H were isolated from the solid culture of an endophytic strain Phaeosphaeriopsis musae M. Arzanlou & Crous (Phaeosphaeriaceae). Their structures were unambiguously elucidated by extensive spectral analyses. The absolute configurations of two diastereomers musaolides G and H were assigned by comparing their experimental and calculated ECD data. Moreover, the in vitro α-glucosidase inhibition and cytotoxic activities of isolated compounds (except musaolide D) were evaluated to understand their biological effects, wherein musaone B and musaolide F displayed promising α-glucosidase inhibition with IC values of 37.3 ± 0.59 μM and 105.18 ± 2.76 μM respectively, compared to the positive control acarbose (24.99 ± 1.28 μM).
从内生真菌 Phaeosphaeriopsis musae M. Arzanlou & Crous（Phaeosphaeriaceae）的固体培养物中分离得到了两个未描述的酚类衍生物 musaones A 和 B、六个未描述的γ-丁内酯 musaolides A-F，以及两个未描述的环戊酮 musaolides G 和 H。通过广泛的光谱分析，明确了它们的结构。通过比较两个非对映异构体 musaolide G 和 H 的实验和计算 ECD 数据，确定了它们的绝对构型。此外，还评估了分离化合物（除了 musaolide D 之外）的体外α-葡萄糖苷酶抑制和细胞毒性活性，以了解它们的生物效应，其中 musaone B 和 musaolide F 对α-葡萄糖苷酶的抑制作用具有很大的潜力，IC 值分别为 37.3 ± 0.59 μM 和 105.18 ± 2.76 μM，与阳性对照阿卡波糖（24.99 ± 1.28 μM）相比。
