Facultad de Ciencias Químicas, Universidad de Concepción, Concepción, Chile.
Facultad de Medicina, Universidad Católica de la Santísima Concepción, Concepción, Chile.
Biophys Chem. 2022 Feb;281:106738. doi: 10.1016/j.bpc.2021.106738. Epub 2021 Dec 9.
The interactions and the protective effect of the carotenoid crocin (CRO) on human erythrocytes (RBC) and molecular models of its membrane were investigated. The latter consisted of bilayers of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), representative of phospholipid classes located in the outer and inner monolayers of the RBC membrane, respectively. X-ray diffraction, differential scanning calorimetry (DSC) and electronic paramagnetic resonance spectroscopy (EPR) showed that CRO produced structural perturbations in DMPC bilayers and in isolated unsealed human erythrocyte membranes. On the other hand, scanning electron microscopy (SEM) showed that CRO induced shape changes in the RBC from their normal discoid form to echinocytes. This result indicates that the CRO molecules were mainly localized in the outer monolayer of the RBC membrane. The assessment of the protective capacity of CRO was revealed by the carotenoid inhibition of the morphological alterations caused by hypochlorous acid (HOCl) to RBC.
研究了类胡萝卜素藏红花素 (CRO) 与人红细胞 (RBC) 及其膜分子模型的相互作用和保护作用。后者由二肉豆蔻酰磷脂酰胆碱 (DMPC) 和二肉豆蔻酰磷脂酰乙醇胺 (DMPE) 的双层组成，分别代表位于 RBC 膜外层和内层单层中的磷脂类。X 射线衍射、差示扫描量热法 (DSC) 和电子顺磁共振波谱 (EPR) 表明 CRO 对 DMPC 双层和分离的未密封人红细胞膜产生了结构扰动。另一方面，扫描电子显微镜 (SEM) 显示 CRO 诱导 RBC 从正常盘状形态变为刺状细胞。这一结果表明 CRO 分子主要定位于 RBC 膜的外层单层。通过类胡萝卜素抑制次氯酸 (HOCl) 引起的 RBC 形态改变，评估了 CRO 的保护能力。
