Department of Exercise Sciences, Shiraz University, Shiraz, Iran.
Department of Internal Medicine, Shiraz University of Medical Sciences, Iran.
Transpl Immunol. 2022 Feb;70:101516. doi: 10.1016/j.trim.2021.101516. Epub 2021 Dec 16.
BACKGROUND: Systemic lupus erythematosus (SLE) is an inflammatory rheumatic disease characterized by production of autoantibodies and organ damage. Elevated levels of cytokines have been reported in SLE patients. Physical activity could be considered one of the factors that affect the immune system status and function. The aim of the present study was to evaluate the effects of an 8-week supervised aerobic and anaerobic training program on the immune system of SLE patients through evaluation of serum cytokine levels. METHODS: This cross-sectional study included 24 SLE patients selected between September 2015 and March 2016. The patients were randomly divided into two groups, including exercise (n = 14) and control (n = 10) groups. The exercise group participated in an 8-week combined supervised exercise training program consisting of three times per week in 60-min exercise sessions. After collection of whole peripheral blood, peripheral blood mononuclear cells (PBMCs) were isolated from the blood samples. Following RNA extraction and cDNA synthesis, the expression levels of IFN-γ, TNF-α, IL-6, IL-2, IL-4, IL-5, IL-9, IL-10, IL-13, IL-17A, IL-17F, IL-21, and IL-22 were determined using in-house SYBER Green-based real-time polymerase chain reaction (PCR). Lastly, the data obtained were analyzed using t-test. RESULTS: The mean and standard deviation of age were 29.00 ± 3.19 and 21.50 ± 5.52 in the intervention and control groups, respectively. No significant differences were found among the mean serum levels of IFN-γ, IL6, IL-9, IL-17A, IL-17F and IL-21 among SLE patients in the intervention and control groups. The mean serum levels of TNF-α, IL2, IL-4, and IL-5 decreased significantly in the intervention as compared with the control group. The mean serum levels of IL-10, IL-13 and IL-22 significantly increased in the control group after eight weeks, as compared with the intervention group. CONCLUSIONS: Our findings indicated that the 8-week supervised aerobic and anaerobic training program could result in decreased inflammatory cytokines.
背景：系统性红斑狼疮（SLE）是一种炎症性风湿性疾病，其特征是产生自身抗体和器官损伤。有报道称，SLE 患者的细胞因子水平升高。身体活动可以被认为是影响免疫系统状态和功能的因素之一。本研究旨在通过评估血清细胞因子水平来评估 8 周监督有氧运动和无氧运动训练计划对 SLE 患者免疫系统的影响。
方法：本横断面研究包括 2015 年 9 月至 2016 年 3 月期间选择的 24 名 SLE 患者。患者随机分为两组，包括运动组（n=14）和对照组（n=10）。运动组参加了 8 周的综合监督运动训练计划，每周 3 次，每次 60 分钟。采集全外周血后，从血液样本中分离外周血单核细胞（PBMC）。提取 RNA 并合成 cDNA 后，使用内部 SYBER 绿色实时聚合酶链反应（PCR）测定 IFN-γ、TNF-α、IL-6、IL-2、IL-4、IL-5、IL-9、IL-10、IL-13、IL-17A、IL-17F、IL-21 和 IL-22 的表达水平。最后，使用 t 检验分析获得的数据。
结果：干预组和对照组的年龄平均值和标准差分别为 29.00±3.19 和 21.50±5.52。干预组和对照组 SLE 患者血清 IFN-γ、IL6、IL-9、IL-17A、IL-17F 和 IL-21 的平均血清水平无显著差异。与对照组相比，干预组 TNF-α、IL2、IL-4 和 IL-5 的平均血清水平显著降低。与干预组相比，对照组 8 周后 IL-10、IL-13 和 IL-22 的平均血清水平显著升高。
结论：我们的研究结果表明，8 周监督有氧运动和无氧运动训练计划可导致炎症细胞因子减少。
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