School of Life Sciences, Anhui University, Hefei, Anhui, 230601, China; Key Laboratory of Human Microenvironment and Precision Medicine of Anhui Higher Education Institutes, Anhui University, Hefei, Anhui, 230601, China.
Institutes of Physical Science and Information Technology, Anhui University, Hefei, Anhui, 230601, China.
Biochem Biophys Res Commun. 2022 Jan 22;589:186-191. doi: 10.1016/j.bbrc.2021.12.022. Epub 2021 Dec 10.
Akkermansia muciniphila is a probiotic that colonizes the outer layer of intestinal mucus and is negatively associated with metabolic disorders. Amuc_2109 protein, a β-N-acetylhexosaminidase from A. muciniphila, may be involved in the degradation of mucins and is associated with intestinal health. Here, we reported the crystal structure of Amuc_2109, which belongs to the GH family 3 enzymes and fell into the canonical (α/β) TIM barrel structure with GlcNAc bound to the active center. Biochemical assay characterization of Amuc_2109 revealed that Amuc_2109 is a GlcNAc-specific glycosidase active over a wide temperature and pH range, reflecting the survival advantage of Amuc_2109 in the intestinal environment. Our structural and biochemical results will contribute to the understanding of the catalytic mechanism of the GH3 β-N-acetylhexosaminidase and help to gain insight into the molecular mechanism of complex carbohydrate utilization and restoration of the intestinal barrier in A. muciniphila.
黏蛋白阿克曼菌是一种定植于肠道黏液外层的益生菌，与代谢紊乱呈负相关。黏蛋白阿克曼菌的 Amuc_2109 蛋白是一种β-N-乙酰氨基葡萄糖苷酶，可能参与黏蛋白的降解，与肠道健康有关。在这里，我们报道了 Amuc_2109 的晶体结构，它属于 GH 家族 3 酶，属于典型的（α/β）TIM 桶结构，GlcNAc 结合在活性中心。Amuc_2109 的生化分析表明，Amuc_2109 是一种在较宽的温度和 pH 范围内具有特异性的 GlcNAc 糖苷酶，反映了 Amuc_2109 在肠道环境中的生存优势。我们的结构和生化结果将有助于理解 GH3 β-N-乙酰氨基葡萄糖苷酶的催化机制，并有助于深入了解复杂碳水化合物利用和黏蛋白阿克曼菌肠道屏障恢复的分子机制。
