Kaminski M S, Kitamura K, Maloney D G, Levy R
J Immunol. 1987 Feb 15;138(4):1289-96.
A murine B cell lymphoma (38C13) was used as a model to study the induction of idiotype (Id)-specific tumor immunity. Immunization of syngeneic mice with Id protein derived from the tumor resulted in the production of anti-Id antibodies by the host and in the induction of a state of resistance to tumor growth. Tumor immunity could be established only if the Id protein was conjugated to a strongly immunogenic carrier protein such as keyhole limpet hemocyanin or thyroglobulin, and if the conjugate was administered at least 1 week prior to tumor challenge. Free Id protein, such as that present in tumor bearing animals, was found to inhibit tumor immunity in a dose-dependent manner. Although tumor immunity could be induced in animals with pre-existent serum Id protein, the expression of the immune state was inhibited by the presence of the soluble protein.
一种小鼠B细胞淋巴瘤（38C13）被用作模型来研究独特型（Id）特异性肿瘤免疫的诱导。用源自肿瘤的Id蛋白免疫同基因小鼠，导致宿主产生抗Id抗体，并诱导出对肿瘤生长的抵抗状态。只有当Id蛋白与强免疫原性载体蛋白（如钥孔血蓝蛋白或甲状腺球蛋白）偶联，并且在肿瘤攻击前至少1周给予偶联物时，才能建立肿瘤免疫。发现游离的Id蛋白（如荷瘤动物体内存在的那种）以剂量依赖的方式抑制肿瘤免疫。尽管在已有血清Id蛋白的动物中可诱导肿瘤免疫，但可溶性蛋白的存在会抑制免疫状态的表达。
