Li Jie, Chen Qiyang, Zhai Xiuming, Wang Dan, Hou Yujia, Tang Min
Research Institute of Tea Chongqing Academy of Agricultural Sciences Chongqing China.
College of Horticulture and Landscape Architecture Southwest University Chongqing China.
Food Sci Nutr. 2021 Oct 6;9(12):6548-6558. doi: 10.1002/fsn3.2580. eCollection 2021 Dec.
Adipose browning leads to increased energy expenditure and reduced adiposity and has, therefore, become an attractive therapeutic strategy for obesity. In this study, we elucidated the effect of green tea aqueous extract (GTAE) on the browning of inguinal white adipose tissue (Ing-WAT) and brown adipose tissue (BAT) in high-fat diet (HFD)-fed mice. The main phytochemical components identified in GTAE through high-performance liquid chromatography (HPLC) included (-)-gallocatechin, (-)-epigallocatechin, (-)-catechin, (-)-epigallocatechin-3-gallate, caffeine, (-)-epicatechin, (-)-gallocatechin gallate, and (-)-epicatechin-3-gallate. Daily supplementation with 1% GTAE for 12 weeks markedly reduced bodyweight gain, systemic inflammation, oxidative stress, and improved insulin resistance. Additionally, histological analysis revealed that dietary supplementation with 1% GTAE reversed HFD-induced adipocyte size and hepatic steatosis. These effects were associated with activation of browning in the Ing-WAT and BAT, which mediate systemic metabolic dysfunction in HFD-fed mice. Taken together, our data support the use of GTAE, a natural product, for the attenuation of obesity through the activation of fat browning.
脂肪褐变会导致能量消耗增加和肥胖程度减轻，因此，已成为一种有吸引力的肥胖治疗策略。在本研究中，我们阐明了绿茶水提取物（GTAE）对高脂饮食（HFD）喂养小鼠腹股沟白色脂肪组织（Ing-WAT）和棕色脂肪组织（BAT）褐变的影响。通过高效液相色谱（HPLC）在GTAE中鉴定出的主要植物化学成分包括（-）-表儿茶素、（-）-表没食子儿茶素、（-）-儿茶素、（-）-表没食子儿茶素-3-没食子酸酯、咖啡因、（-）-表儿茶素、（-）-没食子儿茶素没食子酸酯和（-）-表儿茶素-3-没食子酸酯。每天补充1%GTAE，持续12周，可显著减轻体重增加、全身炎症、氧化应激，并改善胰岛素抵抗。此外，组织学分析显示，饮食中补充1%GTAE可逆转HFD诱导的脂肪细胞大小和肝脂肪变性。这些作用与Ing-WAT和BAT中褐变的激活有关，而褐变在HFD喂养的小鼠中介导全身代谢功能障碍。综上所述，我们的数据支持使用天然产物GTAE通过激活脂肪褐变来减轻肥胖。
