Sparrow R L, Swee-Huat O, Williams N
Leuk Res. 1987;11(1):31-6. doi: 10.1016/0145-2126(87)90102-0.
The biological and immunological properties of stimulators of in vitro murine megakaryocytopoiesis were studied using a heterologous anti-interleukin 3 (IL-3) serum. All megakaryocyte colony development was inhibited with the antiserum using three sources of IL-3, including WEHI-3 cell conditioned medium (WEHI-3CM), pokeweed mitogen-spleen conditioned medium (PWM-SCM) and recombinant IL-3. The data indicate that IL-3 is an absolute requirement for murine megakaryocyte colony development in this system. By comparison the antiserum abolished all myeloid colony growth stimulated by WEHI-3CM, but not PWM-SCM. The in vitro development of single megakaryocytes stimulated by a second putative growth factor, megakaryocyte-potentiator, was not inhibited by the antibody. The antiserum precipitated a 26 kd molecular weight protein from a radioiodinated sample of IL-3. No crossreactivity by the antiserum with other colony-stimulating factors (CSF) including CSF-1 and granulocyte-macrophage CSF was observed. The data indicates that IL-3 and megakaryocyte-potentiator are immunologically unrelated and provides further support that the two factors are separate molecules.
利用一种异源抗白细胞介素3（IL-3）血清，对体外小鼠巨核细胞生成刺激物的生物学和免疫学特性进行了研究。使用三种IL-3来源，包括WEHI-3细胞条件培养基（WEHI-3CM）、商陆有丝分裂原-脾脏条件培养基（PWM-SCM）和重组IL-3，抗血清抑制了所有巨核细胞集落的发育。数据表明，在该系统中，IL-3是小鼠巨核细胞集落发育的绝对必需因子。相比之下，抗血清消除了由WEHI-3CM刺激的所有髓系集落生长，但未消除PWM-SCM刺激的髓系集落生长。由第二种假定的生长因子巨核细胞增强剂刺激的单个巨核细胞的体外发育不受该抗体的抑制。抗血清从IL-3的放射性碘化样品中沉淀出一种分子量为26 kd的蛋白质。未观察到抗血清与其他集落刺激因子（CSF）包括CSF-1和粒细胞-巨噬细胞CSF发生交叉反应。数据表明，IL-3和巨核细胞增强剂在免疫学上不相关，并进一步支持这两种因子是不同的分子。
