Zinchenko V P
Biofizika. 1987 Jan-Feb;32(1):163-4.
Mitogen-induced changes in free Ca2+ concentration in the cytoplasm [Cai2+] of rat thymocytes were studied with the use of quin-2, a Ca2+-sensitive fluorescent indicator. Concanavalin A (Con A) and phytohemagglutinin were shown to increase [Cai2+] from 150 +/- 10 nM for the resting cells up to the value of 380 +/- 10 nM. This increase in [Cai2+] depended on the mitogen concentration. It was observed both in the presence of 1 mM external Ca2+ and in the Ca2+ free medium. The Con A-induced increase of [Cai2+] was not abolished by Na+ removal from the medium or by verapamil, an inhibitor of potential-dependent Ca2+ channels. Hence, the increase in Cai2+ was not due to an activation of potential operated Ca2+-channels. Agents which raise intracellular cAMP blocked Con A-induced increase of [Cai2+].
利用钙离子敏感荧光指示剂喹啉-2研究了有丝分裂原诱导的大鼠胸腺细胞胞质游离钙离子浓度[Ca i 2+]的变化。刀豆球蛋白A（Con A）和植物血凝素可使静息细胞的[Ca i 2+]从150±10 nM升高至380±10 nM。[Ca i 2+]的这种升高依赖于有丝分裂原浓度。在存在1 mM细胞外钙离子和无钙培养基中均观察到这种现象。从培养基中去除钠离子或使用电压依赖性钙离子通道抑制剂维拉帕米均不能消除Con A诱导的[Ca i 2+]升高。因此，[Ca i 2+]的升高并非由于电压门控钙离子通道的激活。提高细胞内cAMP的药物可阻断Con A诱导的[Ca i 2+]升高。
