Winchurch R A, Togo J, Adler W H
Eur J Immunol. 1987 Jan;17(1):127-32. doi: 10.1002/eji.1830170121.
In vitro antibody production to T-dependent erythrocyte antigens is depressed as a function of age. Supplementation of antibody-forming cultures with the essential trace element zinc restores the capacity of cells from immunodepressed, aged mice to generate an antibody response. Zn2+ produces maximal enhancement of immune function when it is added to the cultures within the first 24 h. Supernatants obtained from cultures supplemented with zinc from 0-24 h contain soluble, nondialyzable factors which support enhanced antibody production in fresh cultures of cells from aged mice. Interleukin 2 levels in the supernatants from Zn2+-supplemented cultures were not increased. However, the levels of interleukin 1 were increased approximately 300% over nonsupplemented controls and these increases corresponded with the ability of the supernatants to support antibody formation. Further studies showed that, in addition to enhancing the production of interleukin 1, Zn2+ enhanced the ability of concanavalin A-activated T cells from aged mice to produce B cell stimulatory factor-1.
针对T细胞依赖性红细胞抗原的体外抗体产生能力会随着年龄增长而下降。用必需微量元素锌补充抗体形成培养物，可恢复免疫功能低下的老龄小鼠细胞产生抗体反应的能力。当在最初24小时内将Zn2+添加到培养物中时，它能最大程度地增强免疫功能。在0至24小时内用锌补充的培养物获得的上清液中含有可溶性、不可透析的因子，这些因子能支持老龄小鼠细胞新鲜培养物中抗体产生的增强。补充Zn2+的培养物上清液中的白细胞介素2水平没有增加。然而，与未补充的对照相比，白细胞介素1的水平增加了约300%，并且这些增加与上清液支持抗体形成的能力相对应。进一步研究表明，除了增强白细胞介素1的产生外，Zn2+还增强了老龄小鼠伴刀豆球蛋白A激活的T细胞产生B细胞刺激因子-1的能力。
