Winchurch R A, Togo J, Adler W H
Department of Surgery, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21224.
Clin Immunol Immunopathol. 1988 Nov;49(2):215-22. doi: 10.1016/0090-1229(88)90111-0.
The effects of zinc on interleukin-2(IL-2)-dependent T cell responses of lymphocytes from immunodepressed aged mice and from young adult animals were studied. Concentrations of zinc which have been shown to restore antibody formation in cells from aged mice and to increase the production of Il-1 and Il-4 inhibited the production of Il-2. Cells from both young adults and aged mice were inhibited similarly. Zinc also impaired the ability of aged T cells to proliferate in response to concanavalin A and exogenous Il-2, but enhanced the proliferation of similarly activated splenic cultures containing both T and B cells. Cultures of isolated B lymphocytes produced antibody to sheep red blood cells if the cells were provided with supplemental zinc and Il-1. In contrast, recombinant Il-2 with or without zinc did not activate antibody formation. The results support the premise that the restorative effects of zinc are independent of Il-2 and that Il-2 is not a necessary mediator for antibody production in the aged.
研究了锌对免疫抑制老年小鼠和年轻成年动物淋巴细胞中白细胞介素-2（IL-2）依赖性T细胞反应的影响。已证明能恢复老年小鼠细胞中抗体形成并增加IL-1和IL-4产生的锌浓度会抑制IL-2的产生。年轻成年动物和老年小鼠的细胞受到的抑制相似。锌还损害了老年T细胞对伴刀豆球蛋白A和外源性IL-2作出反应而增殖的能力，但增强了含有T细胞和B细胞的同样活化的脾细胞培养物的增殖。如果为分离的B淋巴细胞培养物提供补充锌和IL-1，这些培养物会产生抗绵羊红细胞抗体。相反，无论有无锌，重组IL-2均不能激活抗体形成。这些结果支持这样的前提，即锌的恢复作用独立于IL-2，并且IL-2不是老年动物抗体产生的必要介质。
