Merchav S, Nagler A, Tatarsky I
Exp Hematol. 1987 Feb;15(2):115-8.
Media conditioned by B-lymphocytes of patients with chronic lymphocytic leukemia (B-CLL) induced the formation of multilineage colonies (CFU-GEMM) in cultures of human target marrow cells. Maximum levels of stem cell colony-stimulating activity (CFU-GEMMCSA) were detected by day 3 of leukemic B-cell cultures, remaining constant thereafter. Stimulation of leukemic B-lymphocytes with B-cell mitogens such as Cowan strain Staphylococcus aureus (SAC) and anti-mu enhanced the production of CFU-GEMMCSA by these cells. 3H-thymidine uptake in response to these mitogens was detected only in SAC-stimulated cultures, thereby demonstrating that mitogen-induced enhancement of CFU-GEMMCSA production by leukemic B-lymphocytes is independent of DNA synthesis. Conditioned media of leukemic B-lymphocytes stimulated the formation of human marrow granulocyte-macrophage (GM-CFU) and early erythroid (BFU-E) colonies as well. These observations indicate a role for B-lymphocytes in the regulation of hemopoietic stem cell and progenitor cell growth in vitro.
慢性淋巴细胞白血病（B-CLL）患者的B淋巴细胞条件培养液可诱导人靶骨髓细胞培养物中多系集落（CFU-GEMM）的形成。白血病B细胞培养至第3天可检测到干细胞集落刺激活性（CFU-GEMMCSA）的最高水平，此后保持恒定。用B细胞有丝分裂原如考恩I株金黄色葡萄球菌（SAC）和抗μ刺激白血病B淋巴细胞可增强这些细胞产生CFU-GEMMCSA的能力。仅在SAC刺激的培养物中检测到对这些有丝分裂原的3H-胸腺嘧啶核苷摄取，从而证明有丝分裂原诱导白血病B淋巴细胞产生CFU-GEMMCSA与DNA合成无关。白血病B淋巴细胞的条件培养液也可刺激人骨髓粒-巨噬细胞（GM-CFU）和早期红系（BFU-E）集落的形成。这些观察结果表明B淋巴细胞在体外造血干细胞和祖细胞生长的调节中发挥作用。
