Johnson G R, Barker D C
Exp Hematol. 1985 Mar;13(3):200-8.
Murine embryonic and fetal yolk-sacs, peripheral blood, and livers were assayed for hemopoietic multipotential and progenitor cell content between days 6 and 13 of gestation. Multipotential cells (Mix-CFC), erythroid-committed progenitor cells (BFU-E), and nonerythroid progenitor cells (predominantly GM-CFC) were assayed by their ability to form hemopoietic colonies in vitro when stimulated by pokeweed-mitogen-stimulated spleen-cell-conditioned media (as a source of Multi-CSF) and either human or murine erythropoietin. Late erythroid progenitor cells (CFU-E) were stimulated to form colonies by erythropoietin. Mix-CFC, BFU-E, and nonerythroid cells were first detected on day 8 in yolk-sacs, day 9 in peripheral blood, and day 11 in liver. Maximum absolute numbers of yolk-sac Mix-CFC (182), BFU-E (331), and non-erythroid CFC (1358) occurred at 11 days of gestation. The maximum frequency of peripheral blood mix-CFC (24/10(5) cells) and BFU-E (55/10(5) cells) occurred at ten days of gestation. The absolute numbers of hepatic Mix-CFC, BFU-E, nonerythroid CFC, and CFU-E increased exponentially from 11 to 13 days' gestation. CFU-E were first detected at nine days in peripheral blood, at ten days in yolk-sac, and 11 days in liver and at all ages were equally responsive to erythropoietin. The maximum frequency (151/10(5) cells) of CFU-E in the peripheral blood and the maximum number per yolk-sac (1699) both occurred on day 11 of gestation. In confirmation of previous studies, yolk-sac fluid was found to contain a macrophage colony-stimulating activity. In addition, an activity capable of stimulating fetal liver CFU-E was also detected in yolk-sac fluid. However, no activity (Multi-CSF) capable of stimulating Mix-CFC or BFU-E was detected in either yolk-sac fluid or fetal plasma.
在妊娠第6至13天期间，对小鼠胚胎和胎儿的卵黄囊、外周血及肝脏进行了造血多能干细胞和祖细胞含量的检测。通过在体外受商陆丝裂原刺激的脾细胞条件培养基（作为多集落刺激因子的来源）以及人或小鼠促红细胞生成素刺激时形成造血集落的能力，来检测多能干细胞（混合集落形成细胞，Mix-CFC）、红系定向祖细胞（爆式红系集落形成单位，BFU-E）和非红系祖细胞（主要是粒-巨噬细胞集落形成单位，GM-CFC）。晚期红系祖细胞（红细胞集落形成单位，CFU-E）由促红细胞生成素刺激形成集落。Mix-CFC、BFU-E和非红系细胞最早分别于第8天在卵黄囊中、第9天在外周血中以及第11天在肝脏中被检测到。卵黄囊Mix-CFC（182个）、BFU-E（331个）和非红系CFC（1358个）的最大绝对数量出现在妊娠第11天。外周血混合集落形成细胞（24/10⁵细胞）和BFU-E（55/10⁵细胞）的最大频率出现在妊娠第10天。肝脏中Mix-CFC、BFU-E、非红系CFC和CFU-E的绝对数量从妊娠第11天到第13天呈指数增加。CFU-E最早于第9天在外周血中、第10天在卵黄囊中以及第11天在肝脏中被检测到，并且在所有年龄段对促红细胞生成素的反应均相同。外周血中CFU-E的最大频率（151/10⁵细胞）和每个卵黄囊的最大数量（1699个）均出现在妊娠第11天。正如之前研究所证实的，发现卵黄囊液中含有巨噬细胞集落刺激活性。此外，在卵黄囊液中还检测到一种能够刺激胎儿肝脏CFU-E的活性。然而，在卵黄囊液或胎儿血浆中均未检测到能够刺激Mix-CFC或BFU-E的活性（多集落刺激因子）。
