Myers C D, Kriz M K, Sullivan T J, Vitetta E S
J Immunol. 1987 Mar 15;138(6):1705-11.
Previous studies have demonstrated a marked change in the metabolism of phospholipids (PL) after activation of resting B lymphocytes with anti-immunoglobulin (anti-Ig). In this study we examined PL metabolism in highly purified trinitrophenyl (TNP)-binding B cells after their activation with various forms of TNP-carrier protein. Such cells show similar changes in PL metabolism when stimulated with either antigen or anti-Ig, i.e., increased incorporation of 32PO4 into phosphatidic acid and phosphatidyl inositol (PI) but not phosphatidyl choline, phosphatidyl ethanolamine, or phosphatidyl serine. We have demonstrated that these responses to antigen are TNP-specific and dose-related between 1 and 50 micrograms/ml, producing up to a 2.5-fold stimulation of 32PO4 incorporation into PI. The PL response is also directly related to the density of TNP on the carrier and can be augmented by additional cross-linking of the carrier protein. These data suggest that cross-linking of surface Ig by antigen induces a change in PL metabolism as an early event in B cell activation.
以往的研究表明，用抗免疫球蛋白（抗Ig）激活静止B淋巴细胞后，磷脂（PL）代谢发生显著变化。在本研究中，我们检测了高度纯化的三硝基苯（TNP）结合B细胞在用各种形式的TNP-载体蛋白激活后的PL代谢。当用抗原或抗Ig刺激时，此类细胞在PL代谢上显示出相似的变化，即32PO4掺入磷脂酸和磷脂酰肌醇（PI）增加，但不掺入磷脂酰胆碱、磷脂酰乙醇胺或磷脂酰丝氨酸。我们已证明，这些对抗原的反应是TNP特异性的，且在1至50微克/毫升之间与剂量相关，可使32PO4掺入PI的量增加至2.5倍。PL反应也与载体上TNP的密度直接相关，并且可通过载体蛋白的额外交联而增强。这些数据表明，抗原对表面Ig的交联作为B细胞激活的早期事件，诱导了PL代谢的变化。
