Kriz M K, Vitetta E S, Sullivan T J
J Immunol. 1986 Jul 15;137(2):478-83.
We have examined phospholipid metabolism in murine B lymphocytes stimulated with anti-Ig bound to Sepharose. T cell-depleted splenic B lymphocytes cultured with Sepharose-coupled, affinity-purified goat anti-mouse Ig (GAMIg) increased the incorporation of 32PO4 into phosphatidic acid and phosphatidylinositol within 3 hr and increased [3H]-thymidine uptake at 48 hr. No increase in labeling was observed in phosphatidylethanolamine, phosphatidylcholine, or phosphatidylserine. Based on both negative and positive selection procedures, it was demonstrated that these responses occurred in B lymphocytes. In contrast to the thymidine uptake response of the GAMIg-stimulated B lymphocytes, the phospholipid response did not require the presence of accessory cells or exogenous cytokines. The same selective changes in phospholipid metabolism were observed in neoplastic B lymphocytes (BCL1) after treatment with Sepharose anti-mu, but not with Sepharose anti-Ia or Sepharose normal Ig. The dose-response relationships of 32PO4 incorporation into phosphatidic acid and phosphatidylinositol and [3H] thymidine uptake were nearly identical in BCL1 cells. The results of these experiments indicate that interaction of B lymphocytes with insolubilized anti-Ig results in prompt and selective changes in phospholipid metabolism that appear to be correlated with B lymphocyte proliferation.
我们检测了用结合在琼脂糖珠上的抗 Ig 刺激的小鼠 B 淋巴细胞中的磷脂代谢。用琼脂糖偶联的、亲和纯化的山羊抗小鼠 Ig（GAMIg）培养的 T 细胞耗尽的脾 B 淋巴细胞，在 3 小时内增加了 32PO4 掺入磷脂酸和磷脂酰肌醇的量，并在 48 小时时增加了[3H] - 胸腺嘧啶核苷的摄取。在磷脂酰乙醇胺、磷脂酰胆碱或磷脂酰丝氨酸中未观察到标记增加。基于阴性和阳性选择程序，证明这些反应发生在 B 淋巴细胞中。与 GAMIg 刺激的 B 淋巴细胞的胸腺嘧啶核苷摄取反应相反，磷脂反应不需要辅助细胞或外源性细胞因子的存在。用琼脂糖抗 μ 处理后，在肿瘤性 B 淋巴细胞（BCL1）中观察到相同的磷脂代谢选择性变化，但用琼脂糖抗 Ia 或琼脂糖正常 Ig 处理则未观察到。在 BCL1 细胞中，32PO4 掺入磷脂酸和磷脂酰肌醇以及[3H]胸腺嘧啶核苷摄取的剂量反应关系几乎相同。这些实验结果表明，B 淋巴细胞与不溶性抗 Ig 的相互作用导致磷脂代谢迅速且选择性地发生变化，这似乎与 B 淋巴细胞增殖相关。
