Sexton P M, McKenzie J S, Mason R T, Moseley J M, Martin T J, Mendelsohn F A
Neuroscience. 1986 Dec;19(4):1235-45. doi: 10.1016/0306-4522(86)90137-5.
The distribution of binding sites for calcitonin gene-related peptide (CGRP) in rat brain were studied using in vitro autoradiography. In a radioreceptor assay using [125I]human calcitonin gene-related peptide as the radioligand, with cerebellar cortical membranes, rat calcitonin gene-related peptide had a binding affinity constant of 1.16 +/- 0.23 X 10(10) M-1 and a site concentration of 43.4 +/- 3.4 fmol/mg protein. In this system, human calcitonin gene-related peptide had a binding affinity constant of 3.9 +/- 0.7 X 10(9) M-1 whereas salmon calcitonin was very weak with a binding affinity constant of only 6.8 +/- 4.0 X 10(5) M-1. CGRP binding localized by in vitro autoradiography, using [125I]rat calcitonin gene-related peptide, had a characteristic distinct distribution in the rat brain. There were high concentrations of binding found over the accumbens nucleus, the organum vasculosum of the lamina terminalis, ventral caudate putamen, median eminence, the arcuate nucleus, lateral amygdaloid nucleus and lateral mammillary nucleus, the superior and inferior colliculi, pontine nuclei, molecular and Purkinje cell layers of the cerebellar cortex, the nucleus of the solitary tract, the inferior olivary nuclei, hypoglossal complex and the vestibular and cochlear nuclei. The distribution of these binding sites suggests multiple roles for CGRP in the central nervous system including auditory, visual, gustatory and somatosensory processing, and in neuroendocrine control.
采用体外放射自显影法研究了大鼠脑中降钙素基因相关肽（CGRP）结合位点的分布。在以[125I]人降钙素基因相关肽为放射性配体的放射受体分析中，对于小脑皮质膜，大鼠降钙素基因相关肽的结合亲和力常数为1.16±0.23×10(10)M-1，位点浓度为43.4±3.4fmol/mg蛋白。在该系统中，人降钙素基因相关肽的结合亲和力常数为3.9±0.7×10(9)M-1，而鲑鱼降钙素的结合亲和力常数非常低，仅为6.8±4.0×10(5)M-1。使用[125I]大鼠降钙素基因相关肽通过体外放射自显影定位的CGRP结合在大鼠脑中具有独特的分布特征。在伏隔核、终板血管器、腹侧尾状壳核、正中隆起、弓状核、外侧杏仁核和外侧乳头体核、上丘和下丘、脑桥核、小脑皮质的分子层和浦肯野细胞层、孤束核、下橄榄核、舌下神经复合体以及前庭核和蜗神经核中发现有高浓度的结合。这些结合位点的分布表明CGRP在中枢神经系统中具有多种作用，包括听觉、视觉、味觉和躯体感觉处理以及神经内分泌控制。
