Skofitsch G, Jacobowitz D M
Peptides. 1985 Sep-Oct;6(5):975-86. doi: 10.1016/0196-9781(85)90331-6.
Using autoradiographic method and 125I-Tyro rat CGRP as a ligand, receptor binding sites were demonstrated in the rat central nervous system. Saturation studies and Scatchard analysis of CGRP-binding to slide mounted tissue sections containing primarily cerebellum showed a single class of receptors with a dissociation constant of 0.96 nM and a Bmax of 76.4 fmol/mg protein. 125I-Tyro rat CGRP binding sites were demonstrated throughout the rat central nervous system. Dense binding was observed in the telencephalon (medial prefrontal, insular and outer layers of the temporal cortex, nucleus accumbens, fundus striatum, central and inferior lateral amygdaloid nuclei, most caudal caudate putamen, organum vasculosum laminae terminalis, subfornical organ), the diencephalon (anterior hypothalamic, suprachiasmatic, arcuate, paraventricular, dorsomedial, periventricular, reuniens, rhomboid, lateral thalamic pretectalis and habenula nuclei, zona incerta), in the mesencephalon (superficial layers of the superior colliculus, central nucleus of the geniculate body, inferior colliculus, nucleus of the fifth nerve, locus coeruleus, nucleus of the mesencephalic tract, the dorsal tegmental nucleus, superior olive), in the molecular layer of the cerebellum, in the medulla oblongata (inferior olive, nucleus tractus solitarii, nucleus commissuralis, nuclei of the tenth and twelfth nerves, the prepositus hypoglossal and the gracilis nuclei, dorsomedial part of the spinal trigeminal tract), in the dorsal gray matter of the spinal cord (laminae I-VI) and the confines of the central canal. Moderate receptor densities were found in the septal area, the "head" of the anterior caudate nucleus, medial amygdaloid and bed nucleus of the stria terminalis, the pyramidal layers of the hippocampus and dentate gyri, medial preoptic area, ventromedial nucleus, lateral hypothalamic and ventrolateral thalamic area, central gray, reticular part of the substantia nigra, parvocellular reticular nucleus. Purkinje cell layer of the cerebellum, nucleus of the spinal trigeminal tract and gracile fasciculus of the spinal cord. The discrete distribution of CGRP-like binding sites in a variety of sensory systems of the brain and spinal cord as well as in thalamic and hypothalamic areas suggests a widespread involvement of CGRP in a variety of brain functions.
采用放射自显影法，以125I - 酪氨酸大鼠降钙素基因相关肽（CGRP）作为配体，在大鼠中枢神经系统中证实了受体结合位点。对主要包含小脑的载玻片组织切片进行CGRP结合的饱和研究和Scatchard分析显示，存在一类单一的受体，其解离常数为0.96 nM，最大结合容量（Bmax）为76.4 fmol/mg蛋白质。在整个大鼠中枢神经系统中均证实了125I - 酪氨酸大鼠CGRP结合位点。在端脑（内侧前额叶、岛叶和颞叶皮质外层、伏隔核、纹状体底部、中央和外侧杏仁核下部、最尾侧的尾状壳核、终板血管器、穹窿下器）、间脑（下丘脑前部、视交叉上核、弓状核、室旁核、背内侧核、室周核、连合核、菱形核、外侧丘脑前顶盖和缰核、未定带）、中脑（上丘浅层、外侧膝状体中央核、下丘、三叉神经核、蓝斑、中脑导水管周围灰质、背侧被盖核、上橄榄核）、小脑分子层、延髓（下橄榄核、孤束核、连合核、第十和第十二脑神经核、舌下前置核和薄束核、脊髓三叉神经束背内侧部）、脊髓背侧灰质（I - VI层）以及中央管边界观察到密集结合。在隔区、尾状核前部“头部”、内侧杏仁核和终纹床核、海马和齿状回的锥体细胞层、内侧视前区、腹内侧核、外侧下丘脑和腹外侧丘脑区、中央灰质、黑质网状部、小细胞网状核、小脑蒲肯野细胞层、脊髓三叉神经核和脊髓薄束中发现中等受体密度。CGRP样结合位点在脑和脊髓的多种感觉系统以及丘脑和下丘脑区域的离散分布表明，CGRP广泛参与多种脑功能。
