Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, 50200, Thailand.
Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, 50200, Thailand.
Chem Biodivers. 2022 Feb;19(2):e202100799. doi: 10.1002/cbdv.202100799. Epub 2022 Jan 13.
This study aimed to revealed anti-inflammatory and antimicrobial activities of fermented Ocimum sanctum Linn. (FE). The fermentation process with Lactobacillus plantarum was compared with the solvent extraction methods. Antimicrobial activity against the growth of Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acnes, Candida albicans, and Malassezia furfur was investigated via broth dilution method. High performance thin layer chromatography was used to determine eugenol content. The anti-inflammation was investigated by means of nuclear factor kappa B (NF-κB) expression inhibition by Western blot analysis. FE yielded the highest amount (11.93 % w/w), the highest eugenol content (39.3±12.6 % w/w), and the highest antimicrobial activities comparing to the extracts obtained from the solvent extractions. The fungal inhibition against M. furfur 656 was equivalent to that of ketoconazole. Furthermore, the bacterial inhibition on S. aureus and S. epidermidis was compared to that of Penicillin G at minimum inhibitory concentration (MIC) of 0.125 mg/mL and 0.25 mg/mL, respectively. Interestingly, FE had lower MIC and minimum bactericidal concentration against P. acnes than Penicillin G and also possessed comparable anti-inflammatory activity to indomethacin with the NF-κB suppression of 42.7±4.6 %. Therefore, FE are potentially natural anti-inflammation and antimicrobial agents for topical applications in the pharmaceutical and cosmetic industries.
本研究旨在揭示发酵神圣罗勒（FE）的抗炎和抗菌活性。用植物乳杆菌进行发酵的过程与溶剂提取方法进行了比较。采用肉汤稀释法研究了对金黄色葡萄球菌、表皮葡萄球菌、痤疮丙酸杆菌、白色念珠菌和糠秕马拉色菌生长的抗菌活性。采用高效薄层色谱法测定丁香酚含量。通过 Western blot 分析抑制核因子 kappa B（NF-κB）表达来研究抗炎作用。FE 的产量最高（11.93%w/w），丁香酚含量最高（39.3±12.6%w/w），抗菌活性最高，与溶剂提取物相比。对糠秕马拉色菌 656 的真菌抑制作用与酮康唑相当。此外，在最低抑菌浓度（MIC）为 0.125 mg/mL 和 0.25 mg/mL 时，FE 对金黄色葡萄球菌和表皮葡萄球菌的抑菌作用分别与青霉素 G 相当，并且在 NF-κB 抑制方面具有与消炎痛相当的抗炎活性，为 42.7±4.6%。因此，FE 可能是用于制药和化妆品行业局部应用的天然抗炎和抗菌剂。
