Srichok Janejira, Yingbun Natthika, Kowawisetsut Teerada, Kornmatitsuk Sudsaijai, Suttisansanee Uthaiwan, Temviriyanukul Piya, Chantong Boonrat
Faculty of Veterinary Science, Mahidol University, Salaya, Phutthamonthon, Nakhon Pathom 73170, Thailand.
Department of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom 73170, Thailand.
Antibiotics (Basel). 2022 Apr 12;11(4):510. doi: 10.3390/antibiotics11040510.
Mastitis is the most prevalent global illness affecting dairy cows. This bacterial infection damages and inflames the udder tissues. Several plant extracts have demonstrated synergistic antibacterial activities with standard drugs in mastitis treatment. Scant information exists on L. This study evaluated the antibacterial activity of extract and its interaction with antibacterial drugs against common mastitis pathogens including , coagulase-negative Staphylococci (CNS), , and . Anti-inflammatory activities in LPS-stimulated RAW264.7 macrophage cells were also studied. The extract exhibited antibacterial activities against , CNS, and with minimum inhibitory concentration (MIC) ranging from 3.9 to 31.2 µg/mL and minimum bactericidal concentration (MBC) ranging from 15.6 to 500 µg/mL. Combinations of with penicillin or amoxicillin-clavulanic acid showed synergistic effects against all tested strains but an additive effect with cefazolin and gentamicin. Pretreatment of the extract significantly decreased the expression of inflammatory molecules (IL-6, TNF-α, IL-1β, iNOS, COX-2, and PGE2) generated by LPS in macrophages. Results suggested effectiveness against various Gram-positive mastitis bacteria, with the potential to reduce antibacterial doses and combat inflammation.
乳腺炎是影响奶牛的全球最普遍疾病。这种细菌感染会损害并炎症乳腺组织。几种植物提取物在乳腺炎治疗中已显示出与标准药物的协同抗菌活性。关于[提取物名称]的信息很少。本研究评估了[提取物名称]提取物的抗菌活性及其与抗菌药物对包括[细菌名称1]、凝固酶阴性葡萄球菌（CNS）、[细菌名称2]和[细菌名称3]在内的常见乳腺炎病原体的相互作用。还研究了其在脂多糖刺激的RAW264.7巨噬细胞中的抗炎活性。[提取物名称]提取物对[细菌名称1]、CNS和[细菌名称2]表现出抗菌活性，最低抑菌浓度（MIC）范围为3.9至31.2μg/mL，最低杀菌浓度（MBC）范围为15.6至500μg/mL。[提取物名称]与青霉素或阿莫西林 - 克拉维酸的组合对所有测试菌株均显示出协同作用，但与头孢唑林和庆大霉素的组合表现为相加作用。提取物预处理显著降低了脂多糖在巨噬细胞中产生的炎症分子（IL - 6、TNF - α、IL - 1β、iNOS、COX - 2和PGE2）的表达。结果表明[提取物名称]对各种革兰氏阳性乳腺炎细菌有效，具有降低抗菌剂量和对抗炎症的潜力。
