Analysis & Testing Laboratory, Xinxiang Medical University, Jinsui Road 601 Xinxiang, Henan, 453003, China.
School of Pharmacy, Xinxiang, Medical University, Jinsui Road 601 Xinxiang, Henan, 453003, China.
Chem Biodivers. 2022 Feb;19(2):e202100862. doi: 10.1002/cbdv.202100862. Epub 2022 Jan 13.
We reported the specific fluorescent probe (MC-BOD-XDS) with two-steps reaction based on monosulfanyl-coumarin-BODIPY for selective detection of cysteine, high activity sulfanyl-coumarin as the multiple reaction group instead of a group internal standard fluorophore. The reaction mechanism of MC-BOD-XDS for detecting cysteine was different from the reported probes about the nucleophilic aromatic substitution reaction (SNAr) of chlorinated BODIPY. The fluorescent color of MC-BOD-XDS changed from yellow to red, and then to orange. The linear calibration diagram showed that it can potentially be used for quantitatively detection of Cys. Its potential applications were demonstrated by employing it for detection of Cys in artificial urine and in fluorescent imaging in HeLa cells.
我们报道了一种基于单硫代香豆素-BODIPY 的两步反应的特定荧光探针 (MC-BOD-XDS)，用于选择性检测半胱氨酸，高反应性的硫代香豆素作为多个反应基团，而不是基团内标准荧光团。MC-BOD-XDS 检测半胱氨酸的反应机制与报道的关于氯化 BODIPY 的亲核芳香取代反应 (SNAr) 的探针不同。MC-BOD-XDS 的荧光颜色从黄色变为红色，然后变为橙色。线性校准图表明，它可能可用于半胱氨酸的定量检测。通过将其用于人工尿液中的半胱氨酸检测和 HeLa 细胞中的荧光成像，证明了其潜在的应用。
