Wong G G, Temple P A, Leary A C, Witek-Giannotti J S, Yang Y C, Ciarletta A B, Chung M, Murtha P, Kriz R, Kaufman R J
Science. 1987 Mar 20;235(4795):1504-8. doi: 10.1126/science.3493529.
A 4-kilobase complementary DNA (cDNA) encoding human macrophage-specific colony-stimulating factor (CSF-1) was isolated. When introduced into mammalian cells, this cDNA directs the expression of CSF-1 that is structurally and functionally indistinguishable from the natural human urinary CSF-1. Direct structural analysis of both the recombinant CSF-1 and the purified human urinary protein revealed that these species contain a sequence of at least 40 amino acids at their carboxyl termini which are not found in the coding region of a 1.6-kilobase CSF-1 cDNA that was previously described. These results demonstrate that the human CSF-1 gene can be expressed to yield at least two different messenger RNA species that encode distinct but related forms of CSF-1.
分离出了一段编码人巨噬细胞特异性集落刺激因子（CSF-1）的4千碱基互补DNA（cDNA）。当将此cDNA导入哺乳动物细胞时，它指导CSF-1的表达，该表达产物在结构和功能上与天然人尿CSF-1无法区分。对重组CSF-1和纯化的人尿蛋白进行的直接结构分析表明，这些蛋白在其羧基末端含有至少40个氨基酸的序列，而该序列在先前描述的1.6千碱基CSF-1 cDNA的编码区中未发现。这些结果表明，人CSF-1基因可以表达产生至少两种不同的信使RNA，它们编码不同但相关形式的CSF-1。
