Kawasaki E S, Ladner M B, Wang A M, Van Arsdell J, Warren M K, Coyne M Y, Schweickart V L, Lee M T, Wilson K J, Boosman A
Science. 1985 Oct 18;230(4723):291-6. doi: 10.1126/science.2996129.
Complementary DNA (cDNA) clones encoding human macrophage-specific specific colony-stimulating factor (CSF-1) were isolated. One cDNA clone codes for a mature polypeptide of 224 amino acids and a putative leader of 32 amino acids. This cDNA, which was cloned in the Okayama-Berg expression vector, specifies the synthesis of biologically active CSF-1 in COS cells, as determined by a specific radioreceptor assay, macrophage bone marrow colony formation, and antibody neutralization. Most of the cDNA isolates contain part of an intron sequence that changes the reading frame, resulting in an abrupt termination of translation; these cDNA's were inactive in COS cells. The CSF-1 appears to be encoded by a single-copy gene, but its expression results in the synthesis of several messenger RNA species, ranging in size from about 1.5 to 4.5 kilobases.
编码人巨噬细胞特异性集落刺激因子（CSF-1）的互补DNA（cDNA）克隆被分离出来。一个cDNA克隆编码一个由224个氨基酸组成的成熟多肽和一个推测的由32个氨基酸组成的前导序列。这个克隆于冈山-伯格表达载体中的cDNA，通过特异性放射受体分析、巨噬细胞骨髓集落形成和抗体中和试验确定，在COS细胞中能指导具有生物活性的CSF-1的合成。大多数cDNA分离物包含一个改变阅读框的内含子序列的一部分，导致翻译突然终止；这些cDNA在COS细胞中无活性。CSF-1似乎由一个单拷贝基因编码，但其表达导致几种大小约为1.5至4.5千碱基的信使RNA的合成。
