Department of Organic Chemistry, Faculty of Sciences, Universidad Autónoma de Madrid, Madrid, Spain.
Department of Pharmacology, Pharmacognosy and Botany, Faculty of Pharmacy, Universidad Complutense de Madrid, Madrid, Spain.
Nat Prod Res. 2022 Nov;36(22):5803-5807. doi: 10.1080/14786419.2021.2016751. Epub 2021 Dec 22.
commonly known as Mashua, is an herbal remedy used on the skin in order to treat local pain and to heal wounds. This study aimed to evaluate the extracts and isolated compounds from with anti-glycative and anti-inflammatory activities. Guided isolation by bioassay led to the isolation and characterisation by NMR and MS of ()-(-)--(-methylbenzyl)-oleamide ) and ()-(-)--(-methylbenzyl)-linoleamide (). Both compounds inhibited the production of TNF-α with IC values of 9.38 µM (NIH/3T3 cells) and 10.06 µM (PA317 cells) for compound , and 5.3 µM (NIH/3T3 cells) and 6.48 µM (PA317 cells) for compound . Compounds and showed the inhibitory effect on the BSA-MGO formation at concentrations of 9.38 µM (3.39%) and 5.30 µM (8.53%), respectively. Moreover, both compounds showed significant breaking properties on the MGO-AGE-protein crosslink with percent modification of 6.58% (9.38 µM) and 18.08% (5.30 µM), respectively.
玛树，是一种被广泛认知的草药，通常被用于治疗局部疼痛和伤口愈合。本研究旨在评估玛树的提取物和分离化合物的抗糖化和抗炎活性。通过生物测定指导的分离，得到了()-(-)--(-甲基苄基)-油酸酰胺 ()和()-(-)--(-甲基苄基)-亚油酸酰胺 ()。这两种化合物都抑制了 TNF-α的产生，其 IC 值分别为 9.38 μM（NIH/3T3 细胞）和 10.06 μM（PA317 细胞）（化合物 ），5.3 μM（NIH/3T3 细胞）和 6.48 μM（PA317 细胞）（化合物 ）。化合物 和 在 9.38 μM（3.39%）和 5.30 μM（8.53%）浓度下，均表现出抑制 BSA-MGO 形成的作用。此外，这两种化合物均表现出对 MGO-AGE-蛋白交联的显著断裂特性，其修饰百分比分别为 6.58%（9.38 μM）和 18.08%（5.30 μM）。
