Alabbasi Samah F, Viramontes Ariel C, Diaz Francisco J, Weedn Victor W
From the Department of Forensic Sciences, George Washington University.
Department of Pathology and Laboratory Medicine, MedStar Georgetown University Hospital.
Am J Forensic Med Pathol. 2022 Jun 1;43(2):142-146. doi: 10.1097/PAF.0000000000000739. Epub 2021 Dec 21.
The loss of basophilia (LOB), as an objective marker of postmortem interval (PMI), was evaluated. Such a correlation has been previously reported in stillborn fetuses.
Loss of basophilia in different tissues was scored using hematoxylin and eosin-stained slides obtained from 65 random autopsy cases. Scatter plots were used to visually assess the correlation of PMI with our LOB scores. Decomposition was assessed using a modified total body score.
Loss of basophilia was found to be correlated with PMI (total and unrefrigerated intervals). Specifically in this study, we found full or partial basophilic staining up to 26 hours after death, and complete LOB was seen in cases as early as 36 hours in liver and 60 hours in heart. Loss of basophilia also well correlated with the modified total body score. The LOB varied by tissue and was uncorrelated to histologically observable bacteria and fungi. Refrigeration appeared to stop the autolytic process that causes the LOB.
Complete LOB can be expected between 1 and 2 days after death in unrefrigerated liver and heart tissues because of autolysis.
对嗜碱性粒细胞减少（LOB）作为死后间隔时间（PMI）的客观标志物进行了评估。此前在死产胎儿中已有此类相关性的报道。
使用从65例随机尸检病例获取的苏木精和伊红染色切片，对不同组织中的嗜碱性粒细胞减少情况进行评分。采用散点图直观评估PMI与我们的LOB评分之间的相关性。使用改良的全身评分评估尸体分解情况。
发现嗜碱性粒细胞减少与PMI（总间隔时间和未冷藏间隔时间）相关。具体而言，在本研究中，我们发现在死亡后26小时内存在全部或部分嗜碱性染色，在肝脏中最早于36小时、在心脏中最早于60小时出现完全的LOB。嗜碱性粒细胞减少也与改良的全身评分密切相关。LOB因组织而异，且与组织学上可观察到的细菌和真菌无关。冷藏似乎能阻止导致LOB的自溶过程。
由于自溶作用，在未冷藏的肝脏和心脏组织中，预计在死亡后1至2天会出现完全的LOB。
